Anti-Histone H3 (phospho T32) antibody
| Name | Anti-Histone H3 (phospho T32) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab4076 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P WB ICC/IF |
| Species Reactivities | Human, Arabidopsis thaliana, Mouse, Rat, Chicken, Bovine, Xenopus, Drosophila, Zebrafish |
| Antigen | Synthetic peptide corresponding to Human Histone H3 aa 1-100 (phospho T32) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Blocking Peptide | Human Histone H3 (phospho T32) peptide |
| Description | Rabbit Polyclonal |
| Gene | H3F3A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Hela cells cultured on coverslips were fixed in 4% paraformaldehyde and stained with ab4076 (green) at a working dilution of 1/200. The DNA stainined with DAPI is shown in red. (100x magnification).
All lanes : Anti-Histone H3 (phospho T32) antibody (ab4076) at 1 µg/mlLane 1 : Colcemid treated histone calf thymus lysateLane 2 : Untreated histone calf thymus lysateLane 3 : Colcemid treated histone calf thymus lysate with Human Histone H3 (phospho T32) peptide (ab14799) at 1 µg/mlLane 4 : Untreated calf thymus histone lysate with Human Histone H3 (phospho T32) peptide (ab14799) at 1 µg/mlLane 5 : Colcemid treated calf thymus histone lysate with Human Histone H3 (unmodified ) peptide (ab2623) at 1 µg/mlLane 6 : Untreated calf thymus histone lysate with Human Histone H3 (unmodified ) peptide (ab2623) at 1 µg/mlLysates/proteins at 20 µg per lane.
The antibody gave an excellent signal with the expected pattern in both mitotic and meiotic cells of Arabidopsis thaliana and Secale cereale (see Caperta et al. Cytogenet Genome Res 2008 122, 73-79. S. Karger AG, Basel) Figure: Thr32 phosphorylation of histone H3 (H3T32ph) correlates with mitotic and meiotic condensation in Arabidopsis thaliana and Secale cereale. DAPI stained chromosomes are blue, H3T32ph-signals are red, and the FISH signals corresponding to the A. thaliana 180-bp centromeric repeat are green. At late prophase H3T32ph-signals coincide with entire lengths of chromosomes and the same is observed at metaphase. In diakinesis phosphorylated T32 is dispersed along bivalent arms. In the second metaphase of meiosis, the immunosignals are spread along chromosome arms. Bars=10µm.See Abreview
ab4076 (4µg/ml) staining Histone H3 in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
beta-N-Acetylglucosamine (O-GlcNAc) is a novel regulator of mitosis-specific - beta-N-Acetylglucosamine (O-GlcNAc) is a novel regulator of mitosis-specific
Fong JJ, Nguyen BL, Bridger R, Medrano EE, Wells L, Pan S, Sifers RN. J Biol Chem. 2012 Apr 6;287(15):12195-203.
Distribution patterns of phosphorylated Thr 3 and Thr 32 of histone H3 in plant - Distribution patterns of phosphorylated Thr 3 and Thr 32 of histone H3 in plant
Caperta AD, Rosa M, Delgado M, Karimi R, Demidov D, Viegas W, Houben A. Cytogenet Genome Res. 2008;122(1):73-9.
Distribution patterns of phosphorylated Thr 3 and Thr 32 of histone H3 in plant - Distribution patterns of phosphorylated Thr 3 and Thr 32 of histone H3 in plant
Caperta AD, Rosa M, Delgado M, Karimi R, Demidov D, Viegas W, Houben A. Cytogenet Genome Res. 2008;122(1):73-9.
Chromatin decondensation and nuclear reprogramming by nucleoplasmin. - Chromatin decondensation and nuclear reprogramming by nucleoplasmin.
Tamada H, Van Thuan N, Reed P, Nelson D, Katoku-Kikyo N, Wudel J, Wakayama T, Kikyo N. Mol Cell Biol. 2006 Feb;26(4):1259-71.