H3F3A

Gene Symbol H3F3A
Entrez Gene 326601
Alt Symbol -
Species Bovine
Gene Type protein-coding
Description H3 histone, family 3A
Other Description histone H3.3
Accessions DAA21369 Q5E9F8 AB098793 AB099028 BAC56518 BC134734 AAI34735 BT020962 AAX08979 NM_001014389 NP_001014411
Top Pathways Systemic lupus erythematosus, Alcoholism, Transcriptional misregulation in cancer

Anti-Histone H3 (phospho S28) antibody - ab4178 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
This image was kindly supplied as part of the review submitted by Monika Harrer.

Anti-Histone H3 (phospho S28) antibody [HTA28] - ab10543 from Abcam

Prices $401.00
Sizes 100 µl
Host Rat
Clonality Monoclonal
Clone HTA28
All lanes : Anti-Histone H3 (phospho S28) antibody [HTA28] (ab10543) at 1 µg/mlLane 1 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treatedLane 2 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated with Human Histone H3 (unmodified ) peptide (ab2623) at 0.5 µg/mlLane 3 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated with Human Histone H3 (phospho S28) peptide (ab5499) at 0.5 µg/mlLane 4 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated with Human Histone H3 (phospho S10) peptide (ab11477) at 0.5 µg/mlLysates/proteins at 2.5 µg per lane.SecondaryPeroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 17 kDa (why is the actual band size different from the predicted?)Exposure time : 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto

Anti-Histone H3 (phospho T3) antibody - ab130940 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab130940 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab130940, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488  (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.

Anti-Histone H3 (phospho T32) antibody - ab4076 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Hela cells cultured on coverslips were fixed in 4% paraformaldehyde and stained with ab4076 (green) at a working dilution of 1/200. The DNA stainined with DAPI is shown in red. (100x magnification).

Anti-Histone H3 (phospho T45) antibody - ab26127 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (phospho T45) antibody (ab26127) at 1 µgLane 1 : HeLa treated with Calyculin A for 45min (0nM, untreated)Lane 2 : HeLa treated with Calyculin A for 45min (20nM)Lane 3 : HeLa treated with Calyculin A for 45min (20nM) with Human Histone H3 (phospho T45) peptide (ab26307) at 1 µgLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 (phospho Y41) antibody - ab26310 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (phospho Y41) antibody (ab26310) at 1 µg/mlLane 1 : Hela Whole Cell Lysate - Colcemid Treated Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate Lane 5 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Histone H3 (tri methyl K27, phospho S28) antibody - ab108268 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3 (tri methyl K27, phospho S28) antibody (ab108268) at 1 µg/mlLane 1 : HeLa Histone Preparation Nuclear Lysate at 10 µgLane 2 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µgLane 3 : HeLa Histone Preparation Nuclear Lysate at 10 µg with Human Histone H3 (tri methyl K27, phospho S28) peptide (ab173750) at 1 µg/mlLane 4 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27, phospho S28) peptide (ab173750) at 1 µg/mlLane 5 : HeLa Histone Preparation Nuclear Lysate at 10 µg with Human Histone H3 peptide (ab173779) at 1 µg/mlLane 6 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 peptide (ab173779) at 1 µg/mlLane 7 : HeLa Histone Preparation Nuclear Lysate at 10 µg with Human Histone H3 (tri methyl K27) peptide (ab1782) at 1 µg/mlLane 8 : Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg with Human Histone H3 (tri methyl K27) peptide (ab1782) at 1 µg/mlLane 9 : HeLa Histone

Anti-Histone H3.3 antibody - ab97968 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Histone H3.3 antibody (ab97968) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Anti-Histone H3.3 antibody - ab183902 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Histone H3.3 antibody (ab183902) at 1/10000 dilutionLane 1 : Jurkat whole cell lysateLane 2 : Raji whole cell lysateLane 3 : K562 whole cell lysateLane 4 : THP1 whole cell lysateLane 5 : HL60 whole cell lysateLane 6 : NCI-H929 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Histone H3 antibody - ab70550 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse teratoma tissue labelling Histone H3 with ab70550 at 1/1000 (0.2µg/ml). Detection: DAB.

Anti-Histone H3 antibody - ab137760 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Histone H3 antibody (ab137760) at 1/1000 dilution + IMR32 cell lysate at 30 µg

Anti-Histone H3 antibody - ab128012 from Abcam

Prices $378.00
Sizes 125 µg
Host Sheep
Clonality Polyclonal
IHC image of Histone H3 staining in human colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab128012, 5µg/ml overnight at +4°C. A anti-sheep HRP secondary antibody (ab97130, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

Anti-Histone H3 antibody - ab16061 from Abcam

Prices $376.00
Sizes 100 µg
Host Chicken
Clonality Polyclonal
All lanes : Anti-Histone H3 antibody (ab16061) at 1 µg/mlLane 1 : Calf Thymus Histone Preparation Nuclear Lysate (ab121)Lane 2 : Calf Thymus Histone Preparation Nuclear Lysate (ab121) with Human Histone H3 peptide (ab12149) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Chicken IgY (IRDyeTM 700DX) at 1/10000 dilutionPerformed under reducing conditions.

Histone Core Polyclonal Antibody - PA1-28375 from Thermo Fisher Scientific

Prices $349.00
Sizes 125 µg
Host Sheep
Clonality Polyclonal

Histone H3.3 Polyclonal Antibody - PA5-23288 from Thermo Fisher Scientific

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human small intestine stained using a Histone H3.3 polyclonal antibody (Product # PA5-23288) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Phospho-Acetyl-Histone H3 (Ser10, Lys9) Polyclonal Antibody - PA1-25462 from Thermo Fisher Scientific

Prices $349.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of Phospho-Histone H3 pSer10+acetyl Lys9 in formalin-fixed, paraffin-embedded rat kidney tissue using a Phospho-Histone H3 pSer10+acetyl Lys9 polyclonal antibody (Product # PA1-25462) at a dilution of 0.2 ?g/ml.

Phospho-Histone H3 pSer28 Antibody (HTA28) - MA1-24751 from Thermo Fisher Scientific

Prices $450.00
Sizes 100 µl
Host Rat
Clonality Monoclonal
Clone HTA28
<h6 id="pdp-product-imgtitle"> Phospho-Histone H3 pSer28 Antibody (MA1-24751) </h6><p id="pdp-product-imgdesc"> Immunofluorescent analysis of Phospho-Histone H3 pSer28 in mouse anterior prostate using a Phospho-Histone H3 pSer28 monoclonal antibody (Product # MA1-24751) at a dilution of 1:2000 (red) and a phospho-S6 (pSer235/236) antibody (green). Nuclei were stained using DAPI (blue). </p>

Phospho-Histone H3 (Ser28) Polyclonal Antibody - PA5-17318 from Thermo Fisher Scientific

Prices $309.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of Phospho-Histone H3 pSer28 in postnatal day 1 rat brain using a Phospho-Histone H3 pSer28 polyclonal antibody (Product # PA5-17318) (green). DNA is labeled using a fluorescent blue dye.

Tri-Methyl-Histone H3 (Lys9) Monoclonal Antibody (6F12-H4) - MA5-18065 from Thermo Fisher Scientific

Prices $399.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6F12-H4
Western blot analysis of Tri-Methyl Histone H3 (Lys9) in Hela histones using a Tri-Methyl Histone H3 (Lys9) monoclonal antibody (MA5-18065) at various dilutions. Samples were separated by SDS-PAGE and transferred to a nitrocellulose membrane. The membrane was blocked in 3% NFDM-PBST for 1 hr at room temperature and incubated at 4°C prior to analysis.
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