Anti-Hsp60 antibody [LK-2] (Phycoerythrin)
| Name | Anti-Hsp60 antibody [LK-2] (Phycoerythrin) |
|---|---|
| Supplier | Abcam |
| Catalog | ab82518 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | LK-2 |
| Applications | ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Rabbit, Chicken, Guinea Pig, Hamster, Bovine, Dog, Human, Pig, C. elegans, Monkey, Escherichia coli, Streptococcus |
| Antigen | Recombinant human Hsp60 protein |
| Description | Mouse Monoclonal |
| Gene | HSPD1 |
| Conjugate | Phycoerythrin |
| Supplier Page | Shop |
Product images
ICC/IF image of ab82518 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82518, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow cytometry analysis of 106 Jurkat cells using ab82518 at 1/100 dilution.