Anti-Hsp90 alpha antibody [2G5.G3]
| Name | Anti-Hsp90 alpha antibody [2G5.G3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab79849 |
| Prices | $391.00 |
| Sizes | 200 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 2G5.G3 |
| Applications | FC IHC-F IHC-P ELISA WB IP ICC/IF ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Human Hsp90 alpha |
| Description | Mouse Monoclonal |
| Gene | HSP90AA1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Hsp90 alpha staining in Human Testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79849, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab79849 at 1/100 dilution staining Hsp90 alpha in human keratinocyte cell line HaCaT by Immunocytochemistry/ immunofluorescence. A Fluorophore conjugated goat anti mouse was used as secondary at 1/50 dilution.
![Overlay histogram showing HeLa cells stained with ab79849 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79849, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/5186_Hsp90-alpha-Primary-antibodies-ab79849-8.jpg)
Overlay histogram showing HeLa cells stained with ab79849 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79849, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor - CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor
Al-Alwan LA, Chang Y, Rousseau S, Martin JG, Eidelman DH, Hamid Q. J Immunol. 2014 Aug 1;193(3):1416-26.
Recruitment of a cytoplasmic chaperone relay by the A2A adenosine receptor. - Recruitment of a cytoplasmic chaperone relay by the A2A adenosine receptor.
Bergmayr C, Thurner P, Keuerleber S, Kudlacek O, Nanoff C, Freissmuth M, Gruber CW. J Biol Chem. 2013 Oct 4;288(40):28831-44.
Protein levels of heat shock proteins 27, 32, 60, 70, 90 and thioredoxin-1 in - Protein levels of heat shock proteins 27, 32, 60, 70, 90 and thioredoxin-1 in
Di Domenico F, Sultana R, Tiu GF, Scheff NN, Perluigi M, Cini C, Butterfield DA. Brain Res. 2010 May 28;1333:72-81.