HSP90AA1

Gene Symbol HSP90AA1
Entrez Gene 3320
Alt Symbol EL52, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP-2, LAP2
Species Human
Gene Type protein-coding
Description heat shock protein 90kDa alpha (cytosolic), class A member 1
Other Description HSP 86|LPS-associated protein 2|epididymis luminal secretory protein 52|heat shock 86 kDa|heat shock 90kD protein 1, alpha|heat shock 90kD protein 1, alpha-like 4|heat shock 90kD protein, alpha-like 4|heat shock 90kDa protein 1, alpha|heat shock protein HSP 90-alpha|lipopolysaccharide-associated protein 2|renal carcinoma antigen NY-REN-38
Swissprots Q5CAQ7 Q5CAQ6 B3KPJ9 A8K500 Q9BVQ5 Q2PP14 P07900
Accessions AAA36023 AAA63194 ABC40730 EAW81765 EAW81766 EAW81767 EAW81768 P07900 AF028832 AAC25497 AI250920 AJ890082 CAI64495 AJ890083 CAI64496 AK056446 BAG51711 AK129557 AK291115 BAF83804 AK291607 BAF84296 AK300126 BAG61917 AK310595 BC000987 AAH00987 BC001695 BC007989 AAH07989 BC017233 BC023006 AAH23006 BC108695 AAI08696 BC121062 AAI21063 BX247955 CAD62296 BX248761 CAD66568 DA237149 DC303876 GQ149083 ADD71695 X07270 CAA30255 X15183 CAA33259 XM_011536718 XP_011535020 NM_001017963 NP_001017963 NM_005348 NP_005339
Function Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. {ECO:0000269|PubMed:11274138, ECO:0000269|PubMed:11276205, ECO:0000269|PubMed:15577939, ECO:0000269|PubMed:15937123}.
Subcellular Location Cytoplasm. Melanosome. Cell membrane. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Top Pathways Progesterone-mediated oocyte maturation, Prostate cancer, NOD-like receptor signaling pathway, Protein processing in endoplasmic reticulum, PI3K-Akt signaling pathway

Phospho-HSP90α (Thr5/7) Antibody - 3488 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and COS cells, treated with either λ phosphatase or UV, using Phospho-HSP90α (Thr5/7) Antibody and β-Actin Antibody #4967 as a loading control.

HSP90 (E289) Antibody - 4875 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using HSP90 (E289) Antibody.

HSP90 Antibody - 4874 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells using HSP90 Antibody.

HSP90 (C45G5) Rabbit mAb - 4877 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C45G5
Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells using HSP90 (C45G5) Rabbit mAb.

HSP90α (D1A7) Rabbit mAb - 8165 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1A7
Western blot analysis of extracts from various cell lines using HSP90α (D1A7) Rabbit mAb.

HSP 90 (AC-16) - sc-101494 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

HSP 90 (4F10) - sc-69703 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
HSP 90 (4F10): sc-69703. Western blot analysis of HSP 90 expression in non-transfected: sc-117752 (A) and human HSP 90 transfected: sc-114003 (B) 293T whole cell lysates.

HSP 90α (C-20) - sc-8262 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HSP 90α (C-20) PE: sc-8262 PE. Intracellular FCM analysis of fixed and permeabilized, heat-shocked NIH/3T3 cells. Black line histogram represents the isotype control, normal goat IgG: sc-3992.

HSP 90α/β (F-8) - sc-13119 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
HSP 90α/β (F-8) PE: sc-13119 PE. Intracellular FCM analysis of fixed and permeabilized, heat-shocked NIH/3T3 cells. Black line histogram represents the isotype control, normal mouse IgG<sub>2a</sub>: sc-2867.

HSP 90α (H-41) - sc-382636 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

HSP 90α/β (H-114) - sc-7947 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
HSP 90α/β (H-114): sc-7947. Western blot analysis of human recombinant HSP 90α (A) and human recombinant HSP 90β fusion proteins (B).

HSP 90α/β (N-17) - sc-1055 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
HSP 90α/β (N-17): sc-1055. Western blot analysis of HSP 90α/β expression in untreated (A) and heat shock-activated (B) HeLa whole cell lysates.

HSP 90α/β (S88) - sc-59578 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HSP 90α/β (S88): sc-59578. Western blot analysis of HSP 90 expression in non-transfected 293T: sc-117752 (A), human HSP 90 transfected 293T: sc-114003 (B) and NIH/3T3 (C) whole cell lysates.

HSP 90α/β (AC88) - sc-59577 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
HSP 90α/β (AC88): sc-59577. Western blot analysis of HSP 90α/βexpression in NIH/3T3 whole cell lysate.

Anti-Hsp90 alpha + beta antibody [EPR16621] (Alexa Fluor® 488) - ab202822 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16621
ab202822 staining Hsp90 alpha + beta in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202822 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-Hsp90 alpha + beta antibody [EPR16621] (Alexa Fluor® 647) - ab202823 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16621
ab202823 staining Hsp90 alpha + beta in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202823 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-Hsp90 alpha + beta antibody [EPR16621-67] - ab203126 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16621-67
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Hsp90 alpha + beta with ab203126 at 1/200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasm and nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:-ve control 1: ab203126 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Anti-Hsp90 alpha antibody - ab53110 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Hsp90 alpha antibody (ab53110) at 1/300 dilutionLane 1 : extract from NIH/3T3 cellsLane 2 : extract from NIH/3T3 cells with immunising peptide

Anti-Hsp90 alpha antibody [2G5.G3] - ab79849 from Abcam

Prices $391.00
Sizes 200 µg
Host Mouse
Clonality Monoclonal
Clone 2G5.G3
IHC image of Hsp90 alpha staining in Human Testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79849, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Hsp90 alpha antibody [4F10] - ab48022 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4F10
Anti-Hsp90 alpha antibody [4F10] (ab48022) at 1/1000 dilution + HeLa cell lysate at 20 µgSecondaryGoat anti-mouse secondary antibody conjugated to HRPdeveloped using the ECL technique

Anti-Hsp90 alpha antibody [5G5] - ab128483 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G5
ELISA using ab128483.

Anti-Hsp90 alpha antibody [EPNCIR102] - ab108593 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPNCIR102
All lanes : Anti-Hsp90 alpha antibody [EPNCIR102] (ab108593) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : SKBR3 cell lysateLane 4 : K562 cell lysateLysates/proteins at 10 µg per lane.

Anti-Hsp90 alpha antibody [EPR3953] - ab109248 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3953
All lanes : Anti-Hsp90 alpha antibody [EPR3953] (ab109248) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLysates/proteins at 10 µg per lane.

Anti-Hsp90 alpha antibody [EPR5355] - ab133491 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5355
All lanes : Anti-Hsp90 alpha antibody [EPR5355] (ab133491) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : PC12 cell lysateLane 4 : NIH3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Hsp90 alpha antibody [EPR5355] (Alexa Fluor® 488) - ab193900 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5355
ab93900 staining Hsp90 in HeLa cells. The cells were fixed with 100%methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab193900 at 1/250 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.This product gave a positive signal in 100% methanol (5 min) fixed A549 cells under the same testing conditions.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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