Anti-KAT3A / CBP antibody [CBP5I001]

• Supplier: Abcam
• Catalog: ab50702
• Prices: $385.00
• Sizes: 50 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: CBP5I001
• Applications: DB IHC-P WB IP ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: Recombinant CBP
• Description: Mouse Monoclonal
• Gene: CREBBP
• Conjugate: Unconjugated

Product images

IHC image of KAT3A staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50702, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-KAT3A / CBP antibody [CBP5I001] (ab50702) at 1/30 dilution + RIPA lysate of HeLa cells at 500 µgSecondaryMouse IgG antibody at 1/2500 dilution

Anti-KAT3A / CBP antibody [CBP5I001] (ab50702) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µgSecondaryMouse IgG antibody at 1/2500 dilution

Anti-KAT3A / CBP antibody [CBP5I001] (ab50702) at 1/100 dilution + HeLa whole cell lysate at 50 µgSecondaryMouse IgG antibody at 1/2500 dilution

Staining of HeLa cell lysate with ab50702 at a dilution of 1/5 and Goat anti-mouse secondary at 1/200.

Product References

The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and - The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and

Benedikt A, Baltruschat S, Scholz B, Bursen A, Arrey TN, Meyer B, Varagnolo L, Muller AM, Karas M, Dingermann T, Marschalek R. Leukemia. 2011 Jan;25(1):135-44.