Anti-Ku80 antibody [111]
| Name | Anti-Ku80 antibody [111] |
|---|---|
| Supplier | Abcam |
| Catalog | ab3107 |
| Prices | $384.00 |
| Sizes | 500 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 111 |
| Applications | FC ICC/IF ICC/IF IP WB |
| Species Reactivities | Human, Monkey |
| Antigen | Human B cell nuclei from plasmacytoid 2p68 cells |
| Description | Mouse Monoclonal |
| Gene | XRCC5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab3107 stained HeLa cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3107, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing HeLa cells stained with ab3107 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3107, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/14292_Ku80-Primary-antibodies-ab3107-1.jpg)
Overlay histogram showing HeLa cells stained with ab3107 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3107, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
An inverse switch in DNA base excision and strand break repair contributes to - An inverse switch in DNA base excision and strand break repair contributes to
Sousa MM, Zub KA, Aas PA, Hanssen-Bauer A, Demirovic A, Sarno A, Tian E, Liabakk NB, Slupphaug G. PLoS One. 2013;8(2):e55493.
Host protein Ku70 binds and protects HIV-1 integrase from proteasomal degradation - Host protein Ku70 binds and protects HIV-1 integrase from proteasomal degradation
Zheng Y, Ao Z, Wang B, Jayappa KD, Yao X. J Biol Chem. 2011 May 20;286(20):17722-35.
Comprehensive analysis of the palindromic motif TCTCGCGAGA: a regulatory element - Comprehensive analysis of the palindromic motif TCTCGCGAGA: a regulatory element
Mikula M, Gaj P, Dzwonek K, Rubel T, Karczmarski J, Paziewska A, Dzwonek A, Bragoszewski P, Dadlez M, Ostrowski J. DNA Res. 2010 Aug;17(4):245-60.
Nucleolin associates with the human cytomegalovirus DNA polymerase accessory - Nucleolin associates with the human cytomegalovirus DNA polymerase accessory
Strang BL, Boulant S, Coen DM. J Virol. 2010 Feb;84(4):1771-84.
Coilin interacts with Ku proteins and inhibits in vitro non-homologous DNA end - Coilin interacts with Ku proteins and inhibits in vitro non-homologous DNA end
Velma V, Carrero ZI, Cosman AM, Hebert MD. FEBS Lett. 2010 Dec 1;584(23):4735-9.
PARP-1 transcriptional activity is regulated by sumoylation upon heat shock. - PARP-1 transcriptional activity is regulated by sumoylation upon heat shock.
Martin N, Schwamborn K, Schreiber V, Werner A, Guillier C, Zhang XD, Bischof O, Seeler JS, Dejean A. EMBO J. 2009 Nov 18;28(22):3534-48.
Proteomic analysis of peripheral blood mononuclear cells: selective protein - Proteomic analysis of peripheral blood mononuclear cells: selective protein
Schulz M, Dotzlaw H, Mikkat S, Eggert M, Neeck G. J Proteome Res. 2007 Sep;6(9):3752-9. Epub 2007 Aug 17.