XRCC5

Gene Symbol XRCC5
Entrez Gene 7520
Alt Symbol KARP-1, KARP1, KU80, KUB2, Ku86, NFIV
Species Human
Gene Type protein-coding
Description X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Other Description 86 kDa subunit of Ku antigen|ATP-dependent DNA helicase 2 subunit 2|ATP-dependent DNA helicase II 80 kDa subunit|CTC box-binding factor 85 kDa subunit|CTC85|CTCBF|DNA repair protein XRCC5|Ku autoantigen, 80kDa|Ku86 autoantigen related protein 1|TLAA|X-ray repair cross-complementing protein 5|lupus Ku autoantigen protein p86|nuclear factor IV|thyroid-lupus autoantigen
Swissprots Q9UCQ0 Q4VBQ5 A8K3X5 Q9UCQ1 P13010 Q53HH7 Q7M4N0 Q0Z7V0
Accessions AAC52087 AAD14875 AAY14659 AAY24231 ABG46942 CBX87168 EAW70562 EAW70563 P13010 AK026166 AK096408 AK222603 BAD96323 AK290740 BAF83429 BC019027 AAH19027 BC095442 AAH95442 BG717693 BP375944 BU184024 DQ892660 ABM83586 DQ895925 ABM86851 J04977 AAA59475 M30938 AAA36154 X57500 CAA40736 NM_021141 NP_066964
Function Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'- deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-el
Subcellular Location Nucleus. Nucleus, nucleolus. Chromosome.
Top Pathways Non-homologous end-joining

Ku80 Antibody - 2753 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of lysates from HeLa, A673, A549 and COS cells, using Ku80 antibody.

Ku80 (C48E7) Rabbit mAb - 2180 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C48E7
Western blot analysis of cell extracts from HeLa, A549 and COS cells using Ku80 (C48E7) Rabbit mAb.

Ku-86 (B-1) - sc-5280 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ku-86 (B-1): sc-5280. Western blot analysis of Ku-86 expression in A-431 (A), HeLa (B), MM-142 (C) and KNRK (D) whole cell lysates. Note lack of reactivity in lanes C and D.

Ku-86 (C-20) - sc-1484 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ku-86 (C-20): sc-1484. Western blot analysis of Ku-86 expression in A-431 (A), HeLa (B), C32 (C) and MM-142 (D) whole cell lysates.

Ku-86 (H-300) - sc-9034 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Ku-86 (H-300): sc-9034. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor (A) and immunofluorescence staining of methanol-fixed K-562 cells (B) showing nuclear localization.

Ku-86 (Ku15) - sc-33653 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ku-86 (Ku15): sc-33653. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

Ku-86 (M-20) - sc-1485 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ku-86 (M-20): sc-1485. Western blot analysis of Ku-86 expression in A-431 (A), HeLa (B), C32 (C) and MM-142 (D) whole cell lysates.

Ku-86 (N9C1) - sc-56135 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ku-86 (N9C1): sc-56135. Western blot analysis of Ku-86 expression in K-562 (A), HeLa (B) and C32 (C) whole cell lysates.

Ku-86 (S10B1) - sc-56136 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ku-86 (N3H10): sc-56136. Western blot analysis of Ku-86 expression in K-562 (A), HeLa (B) and C32 (C) whole cell lysates.

Ku-86 (7) - sc-135964 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/500 µl
Host Mouse
Clonality Monoclonal
Ku-86 (7): sc-135964. Western blot analysis of Ku-86 expression in A-431 whole cell lysate.

Ku-86 (5D5.8) - sc-53021 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ku-86 (5D5.8): sc-53021. Western blot analysis of Ku-86 expression in A-431 (A), HeLa (B) and C32 (C) whole cell lysates and A-431 (D), HeLa (E) and C32 (F) nuclear extracts.

Anti-Ku80 (phospho T714) antibody - ab55350 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ku80 (phospho T714) antibody (ab55350) at 1/500 dilutionLane 1 : extracts from COS7 cells with no immunizing peptideLane 2 : extracts from COS7 cells with immunizing peptide

Anti-Ku80 antibody - ab33242 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab33242 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab33242 at 0.5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Ku80 antibody - ab97433 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Ku80 antibody (ab97433) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

Anti-Ku80 antibody - ab55408 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ku80 antibody (ab55408) at 1/500 dilutionLane 1 : COS7 cell extract, without immunizing peptideLane 2 : COS7 cell extract, with immunizing peptide

Anti-Ku80 antibody - ab58469 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab58469 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.

Anti-Ku80 antibody - ab93863 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Ku80 with ab93863 at 1/1000 (1µg/ml). Detection: DAB.

Anti-Ku80 antibody - ab87860 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ku80 antibody (ab87860) at 1 µg/mlLane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Ku80 antibody - ab26026 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-Ku80 antibody - ab48013 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Ku80 antibody (ab48013) at 0.1 µg/ml + HeLa cell lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-Ku80 antibody [111] - ab3107 from Abcam

Prices $384.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone 111
ICC/IF image of ab3107 stained HeLa cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3107, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Ku80 antibody [111] - ab80830 from Abcam

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 111
ICC/IF image of ab80830 stained HEK293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80830, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Ku80 antibody [111] (Biotin) - ab79220 from Abcam

Prices $373.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone 111

Anti-Ku80 antibody [5C5] - ab119935 from Abcam

Prices $398.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5C5
All lanes : Anti-Ku80 antibody [5C5] (ab119935) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : MCF7 cell lysateLane 3 : A549 cell lysateLane 4 : NIH3T3 cell lysate

Anti-Ku80 antibody [5C5] - ab130267 from Abcam

Host Mouse
Clonality Monoclonal
Clone 5C5
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelled with ab130267 antibody at 1/200 dilution with DAB staining.
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