Anti-Lyn antibody [11A7]

• Supplier: Abcam
• Catalog: ab71093
• Prices: $378.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: 11A7
• Applications: ICC/IF ICC/IF WB IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide: NKQQRPVPESQLLPGQRFQT , corresponding to amino acids 39-58 of Human Lyn Run BLAST with Run BLAST with
• Description: Mouse Monoclonal
• Gene: LYN
• Conjugate: Unconjugated

Product images

ab71093 stained RAW 246.7 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71093 at 1/50) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

All lanes : Anti-Lyn antibody [11A7] (ab71093) at 1/2000 dilutionLane 1 : K562 cell lysateLane 2 : Raji cell lysate