LYN

Gene Symbol LYN
Entrez Gene 4067
Alt Symbol JTK8, p53Lyn, p56Lyn
Species Human
Gene Type protein-coding
Description LYN proto-oncogene, Src family tyrosine kinase
Other Description lck/Yes-related novel protein tyrosine kinase|tyrosine-protein kinase Lyn|v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
Swissprots A0AVQ5 P07948
Accessions EAW86764 EAW86765 EAW86766 EAW86767 EAW86768 P07948 AK130135 AK290494 BAF83183 AK298677 BAG60841 BC059394 AAH59394 BC068551 AAH68551 BC075001 AAH75001 BC075002 AAH75002 BC126456 AAI26457 BC126458 AAI26459 BG059526 BU954348 CB990993 CN480554 DA948647 M16038 AAA59540 M79321 AAB50019 XM_011517529 XP_011515831 XM_011517530 XP_011515832 XM_011517531 XP_011515833 XR_928779 NM_001111097 NP_001104567 NM_002350 NP_002341
Function Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. Functions primarily as negative regulator, but can also function as activator, depending on the context. Required for the initiation of the B-cell response, but also for its down- regulation and termination. Plays an important role in the regulation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance. Acts downstream of several immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Plays a role in the inflammatory response to bacterial lipopolysaccharide. Mediates the responses to cytokines and growth factors in hematopoietic progenitors, platelets, erythrocytes, and
Subcellular Location Cell membrane. Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Golgi apparatus. Note=Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. Detected on plasma membrane lipid rafts.
Tissue Specificity Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors. {ECO:0000269|PubMed:3561390, ECO:0000269|PubMed:8064233}.
Top Pathways Fc gamma R-mediated phagocytosis, Epstein-Barr virus infection, Viral carcinogenesis, Chemokine signaling pathway, B cell receptor signaling pathway

Phospho-Lyn (Tyr507) Antibody - 2731 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Ramos cells treated with anti-IgM antibody (12 µg/ml for 2 minutes), using Phospho-Lyn (Tyr507) Antibody. The phospho-specificity of the antibody was characterized by treating the membrane without or with calf intestinal alkaline phosphatase (CIP) after western transfer.

Lyn (C13F9) Rabbit mAb - 2796 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C13F9
Western blot analysis of cell extracts from various cell types using Lyn (C13F9) Rabbit mAb.

Lyn Antibody - 2732 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from Ramos cells treated with anti-IgM antibody (12 µg/ml for 2 minutes) or untreated, using Lyn Antibody.

Lyn (5G2) Mouse mAb - 4576 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 5G2
Western blot analysis of extracts from various cell lines using Lyn (5G2) Mouse mAb.

Lyn (G-7) - sc-376100 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Lyn (G-7): sc-376100. Western blot analysis of Lyn expression in HeLa (A) and Ramos (B) whole cell lysates.

Lyn (2H8D7) - sc-101849 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Lyn (2H8D7): sc-101849. Western blot analysis of Lyn expression in HL-60 (A), L540 (B), SLLP-M2 (C), SEM (D) and Ramos (E) whole cell lysates.

Lyn (H-70) - sc-28790 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Lyn (H-70): sc-28790. Western blot analysis of Lyn expression in Ramos (A) and HL-60 (B) whole cell lysates.

Lyn (44) - sc-15 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Lyn (44): sc-15. Western blot analysis of Lyn expression in HL-60 (A), MCF7 (B), SK-BR-3 (C), 3611-RF (D) and BJAB (E) whole cell lysates.

Lyn (H-6) - sc-7274 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Lyn (H-6): sc-7274. Western blot analysis of Lyn expression in BJAB (A) and HeLa (B) whole cell lysates.

Lyn (42) - sc-136259 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Lyn (42): sc-136259. Western blot analysis of Lyn expression in HEL (human erythroleukemia cell line) whole cell lysate.

Anti-Lyn (phospho Y396) antibody [EP503Y] - ab40660 from Abcam

Prices $391.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP503Y
All lanes : Anti-Lyn (phospho Y396) antibody [EP503Y] (ab40660) at 1/10000 dilutionLane 1 : HeLa cell lysate, non-treated cells.Lane 2 : HeLa cell lysate, cells treated with Pervanadate.

Anti-Lyn (phospho Y507) antibody - ab53122 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Lyn (phospho Y507) antibody (ab53122) at 1/300 dilutionLane 1 : K562 cell extract treated with Hydrogen Peroxide (100uM, 30mins)Lane 2 : K562 cell extract treated with Hydrogen Peroxide (100uM, 30mins) with immunising peptide

Anti-Lyn (phospho Y507) antibody - ab195729 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Lyn (phospho Y507) antibody (ab195729) at 1/500 dilutionLane 1 : H2O2 treated K562 whole cell lysates with antigen-specific peptideLane 2 : H2O2 treated K562 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-Lyn (phospho Y507) antibody [EP504Y] - ab33914 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP504Y
All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/5000 dilutionLane 1 : 10ug Non treated HeLa cell lysateLane 2 : 10ug HeLa cell lysate treated with PervanadateSecondaryGoat anti-rabbit HRP labeled.

Anti-Lyn antibody - ab137338 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Lyn antibody (ab137338) at 1/1000 dilution + A431 whole cell lysate at 30 µg

Anti-Lyn antibody - ab154325 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Lyn antibody (ab154325) at 1/3000 dilution + HepG2 whole cell lysate at 30 µg

Anti-Lyn antibody - ab77400 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-Lyn antibody (ab77400) at 0.1 µg/ml + U937 lysate in RIPA buffer at 35 µg

Anti-Lyn antibody - ab95872 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Lyn antibody (ab95872) at 0.1 µg/mlLane 1 : Ramos cell lysate at 50 µgLane 2 : Ramos cell lysate at 15 µgLane 3 : Ramos cell lysate at 5 µgLane 4 : 293T cell lysate at 50 µg

Anti-Lyn antibody [1174CT22311] - ab191314 from Abcam

Prices $376.00
Sizes 400 µl
Host Mouse
Clonality Monoclonal
Clone 1174CT22311
All lanes : Anti-Lyn antibody [1174CT22311] (ab191314) at 1/1000 dilutionLane 1 : A431 cell line lysateLane 2 : Raji cell line lysateLysates/proteins at 35 µg per lane.SecondaryGoat anti-mouse IgG H&L(HRP) at 1/5000 dilution

Anti-Lyn antibody [11A7] - ab71093 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 11A7
ab71093 stained RAW 246.7 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71093 at 1/50) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Lyn antibody [2H8D7] - ab52563 from Abcam

Host Mouse
Clonality Monoclonal
Clone 2H8D7
All lanes : Anti-Lyn antibody [2H8D7] (ab52563) at 1/2000 dilutionLane 1 : Cell lysates prepared from HL60 cellsLane 2 : Cell lysates prepared from L540 cellsLane 3 : Cell lysates prepared from SLLP-M2 cellsLane 4 : Cell lysates prepared from SEM cellsLane 5 : Cell lysates prepared from Ramos cellsLysates/proteins at 100 µg per lane.SecondaryHRP-conjugated Goat polyclonal to mouse IgG

Anti-Lyn antibody [EPR5019] - ab139186 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5019

Anti-Lyn antibody [LYN-01] - ab1890 from Abcam

Prices $390.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone LYN-01
Central Panel: ab1890 staining Lyn in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.Left-hand panel: Nuclei counterstained with DAPI (blue).Right-hand panel: OverlaySee Abreview

Anti-Lyn antibody [Y497] - ab32398 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y497
Anti-Lyn antibody [Y497] (ab32398) at 1/2000 dilution + Hela cell lysate

Anti-LYN Antibody - LS-C8555 from LifeSpan Bioscience

Prices $445.00
Sizes 250 µg
Host Sheep
Clonality Polyclonal
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