Anti-MDM2 antibody [2A10]
| Name | Anti-MDM2 antibody [2A10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab16895 |
| Prices | $403.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone | 2A10 |
| Applications | ICC/IF ICC/IF WB IP IHC-F IHC-P ICC/IF FC |
| Species Reactivities | Mouse, Human |
| Antigen | Full length MDM2 protein (Human) |
| Description | Mouse Monoclonal |
| Gene | MDM2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab16895 staining MDM2 in MCF7 cells treated with dienogest (ab141282), by ICC/IF. Decrease in MDM2 expression correlates with increased concentration of dienogest, as described in literature.The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab141282 (dienogest) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab16895 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
ab16895 staining MDM2 in MCF7 cells treated with progesterone (ab141252), by ICC/IF. Decrease in MDM2 expression correlates with increased concentration of progesterone, as described in literature.The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab141252 (progesterone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab16895 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
ab16895 (1/200) staining MDM2 in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.See Abreview
![Anti-MDM2 antibody [2A10] (ab16895) at 2 µg/ml + A549 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/20887_Ab16895_1.gif)
Anti-MDM2 antibody [2A10] (ab16895) at 2 µg/ml + A549 cell lysate
ab16895 staining Human normal tonsil. Staining is localised to nuclear + cytoplasmic compartments. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus, at RT: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 min. They were then blocked with Dako Protein block for 10 min (containing casein 0.25% in PBS) , incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 min. Colorimetric detection was completed with DAB for 5 min. Slides were counterstained with Haematoxylin and coverslipped under DePeX.
![Overlay histogram showing HeLa cells stained with ab16895 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16895, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/20889_MDM2-Primary-antibodies-ab16895-5.jpg)
Overlay histogram showing HeLa cells stained with ab16895 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16895, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
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Protein grafting of p53TAD onto a leucine zipper scaffold generates a potent HDM - Protein grafting of p53TAD onto a leucine zipper scaffold generates a potent HDM
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Prognostic value of the hDMP1-ARF-Hdm2-p53 pathway in breast cancer. - Prognostic value of the hDMP1-ARF-Hdm2-p53 pathway in breast cancer.
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DDX31 regulates the p53-HDM2 pathway and rRNA gene transcription through its - DDX31 regulates the p53-HDM2 pathway and rRNA gene transcription through its
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The p53 isoform, Delta133p53alpha, stimulates angiogenesis and tumour - The p53 isoform, Delta133p53alpha, stimulates angiogenesis and tumour
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