MDM2

Gene Symbol MDM2
Entrez Gene 4193
Alt Symbol ACTFS, HDMX, hdm2
Species Human
Gene Type protein-coding
Description MDM2 proto-oncogene, E3 ubiquitin protein ligase
Other Description E3 ubiquitin-protein ligase Mdm2|MDM2 oncogene, E3 ubiquitin protein ligase|Mdm2, p53 E3 ubiquitin protein ligase homolog|Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|double minute 2, human homolog of; p53-binding protein|oncoprotein Mdm2
Swissprots Q13300 Q13299 Q8WYJ2 Q53XW0 Q8WYJ1 Q13297 Q13301 Q13298 Q9UGI3 Q00987 Q13226 A8K2S6 Q9UMT8 Q71TW9 A6NL51
Accessions AAA82061 AAA82237 AAM78554 CAB64448 CAC22426 CAD57166 CAD57167 CAD57168 EAW97201 EAW97202 EAW97203 EAW97204 EAW97205 EAW97206 EAW97207 EAW97208 EAW97209 EAW97210 EAW97211 EAW97212 EAW97213 EAW97214 Q00987 AA708958 AF092843 AAL40178 AF092844 AAL40179 AF092845 AAL40180 AF201370 AAF42995 AF201371 AF385322 AAL13242 AF385323 AAL13243 AF385324 AAL13244 AF385325 AAL13245 AF385326 AAL13246 AF385327 AAL13247 AJ276888 AJ278975 CAC07809 AJ278976 CAC07810 AJ278977 CAC07811 AJ278978 CAC07812 AJ430612 CAD23251 AJ430613 AJ430614 CAD23252 AJ491698 CAD36959 AJ491699 CAD36960 AJ491700 CAD36961 AJ491701 CAD36962 AJ550516 CAD79455 AJ550517 CAD79456 AJ550518 CAD79457 AJ550519 CAD79458 AJ550520 CAD79459 AK290341 BAF83030 AK312105 BAG35041 AM902641 CAP16703 AM902642 CAP16704 AM902643 CAP16705 AM902644 CAP16706 AM902645 CAP16707 AM902646 CAP16708 AM902647 CAP16709 AM902648 CAP16710 AM902649 CAP16711 AM902650 CAP16712 AM902651 CAP16713 AM902652 CAP16714 AM902653 CAP16715 AM902654 CAP16716 AM902655 CAP16717 AM902656 CAP16718 AM902657 CAP16719 AM902658 CAP16720 AM902659 CAP16721 AM902660 CAP16722 AM902661 CAP16723 AM902662 CAP16724 AM902663 CAP16725 AM902664 CAP16726 AM902665 CAP16727 AM902666 CAP16728 AM902667 CAP16729 AM902668 CAP16730 AM902669 CAP16731 AM902670 CAP16732 AM902671 CAP16733 AM902672 CAP16734 AM902673 CAP16735 AM902674 CAP16736 AM902675 CAP16737 AM902676 CAP16738 AM902677 CAP16739 AM902678 CAP16740 AM902679 CAP16741 AM902680 CAP16742 AM902681 CAP16743 BC006795 BC065505 BC107414 BC148522 AAI48523 BC152384 AAI52385 BC152390 AAI52391 BC153117 AAI53118 BE296905 BE930512 BF208689 BT007258 AAP35922 EU076746 ABT17083 EU076747 ABT17084 EU076748 ABT17085 EU076749 ABT17086 GQ848196 ACX31156 HM213901 HM213902 HM213903 HM213904 HY174841 JX015379 AFM80527 JX015380 AFM80528 JX015381 AFM80529 JX015382 AFM80530 JX015383 AFM80531 JX015384 AFM80532 JX015385 AFM80533 JX015386 AFM80534 JX015387 AFM80535 JX015388 AFM80536 JX015389 AFM80537 JX015390 AFM80538 JX015391 AFM80539 JX015392 AFM80540 M92424 AAA60568 U33199 AAA75514 U33200 AAA75515 U33201 AAA75516 U33202 AAA75517 U33203 AAA75518 Z12020 CAA78055 XM_005268872 XP_005268929 XM_006719399 XP_006719462 XM_006719400 XP_006719463 NM_001145337 NP_001138809 NM_001145339 NP_001138811 NM_001145340 NP_001138812 NM_001278462 NP_001265391 NM_002392 NP_002383 NM_001145336 NM_006878 NM_006879 NM_006880 NM_006881 NM_006882 NM_032739
Function E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. {ECO:0000269|PubMed:12821780, ECO:0000269|PubMed:15053880, ECO:0000269|PubMed:15195100, ECO:0000269|PubMed:15632057, ECO:0000269
Subcellular Location Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Note=Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
Tissue Specificity Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
Top Pathways Epstein-Barr virus infection, Proteoglycans in cancer, PI3K-Akt signaling pathway, MicroRNAs in cancer, Cell cycle

Phospho-MDM2 (Ser166) Antibody - 3521 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MCF-7 cells treated with IGF-1 for the indicated times, using Phospho-MDM2 (Ser166) Antibody.

MDM2 (SMP14) - sc-965 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MDM2 (SMP14): sc-965. Western blot analysis of MDM2 expression in U-2 OS (A), A-673 (B) and RAW 264.7 (C) whole cell lysates.

MDM2 (C-18) - sc-812 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MDM2 (C-18): sc-812. Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing cytoplasmic localization.

MDM2 (N-20) - sc-813 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MDM2 (N-20): sc-813. Western blot analysis of MDM2 expression in PMA-treated Jurkat (A) and U-2 OS (B) whole cell lysates.

MDM2 (D-12) - sc-5304 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MDM2 (D-12): sc-5304. Western blot analysis of MDM2 expression in Jurkat whole cell lysate.

MDM2 (H-221) - sc-7918 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MDM2 (H-221): sc-7918. Western blot analysis of MDM2 expression in Jurkat whole cell lysate.

MDM2 (D-7) - sc-13161 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MDM2 (D-7): sc-13161. Western blot analysis of MDM2 expression in MCF7 whole cell lysate.

MDM2 (SPM344) - sc-56430 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MDM2 (SPM344): sc-56430. Western blot analysis of MDM2 expression in U2-OS (A), A-673 (B) and MCF7 (C) whole cell lysates.

MDM2 (HDM2-323) - sc-56154 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
MDM2 (HDM2-323): sc-56154. Western blot analysis of MDM2 expression in Saos-2 whole cell lysate.

p-MDM2 (Ser 166) - sc-293105 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-MDM2 (Ser 166): sc-293105. Western blot analysis of MDM2 phosphorylation expression in untreated (A) and Hydroxyurea treated (B) 293 whole cell lysates.

p-MDM2 (Thr 218) - sc-130199 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-MDM2 (Thr 218): sc-130199. Western blot analysis of MDM2 phosphorylation in Jurkat whole cell lysate.

Anti-MDM2 (phospho S166) antibody - ab131355 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MDM2 (phospho S166) antibody (ab131355) at 1/500 dilutionLane 1 : 293 cell extract untreatedLane 2 : 293 cell extract treated with hydroxyurea

Anti-MDM2 (phospho S166) antibody - ab58532 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MDM2 (phospho S166) antibody (ab58532) at 1/500 dilutionLane 1 : COS7 cell extractLane 2 : COS7 cell extract with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - ab170880 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1450(2)
All lanes : Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880) at 1/50000 dilutionLane 1 : MCF7 cell lysatesLane 2 : MCF7 cell lysates treated with IGF-1Lysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit IgG at 1/2000 dilution

Anti-MDM2 (phospho S185) antibody - ab1094 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MDM2 (phospho S186) antibody [2G2] - ab22710 from Abcam

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2G2
Anti-MDM2 (phospho S186) antibody [2G2] (ab22710) at 1/1000 dilution + Whole cell lysates prepared from Jurkat cells at 100000 cellsSecondaryHRP-conjugated Sheep polyclonal to Mouse IgG at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MDM2 (phospho S188 + S186) antibody - ab111617 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab111617 at 1/100 dilution staining MDM2 in HeLa cells by Immunofluorescence. The picture on the right is treated with the synthesized phosphopeptide.

Anti-MDM2 antibody - ab58530 from Abcam

Host Rabbit
Clonality Polyclonal
MDM2 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Rabbit polyclonal to MDM2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab58530.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 85kDa; MDM2

Anti-MDM2 antibody - ab137413 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling MDM2 with ab137413 at 1/500 (Green). Alpha-tubulin filaments were also labelled (red).

Anti-MDM2 antibody - ab53710 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MDM2 antibody (ab53710) at 1/500 dilutionLane 1 : Extracts from SKOV3 cells, minus immunising peptideLane 2 : Extracts from SKOV3 cells, plus immunising peptide

Anti-MDM2 antibody - ab87134 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-MDM2 antibody (ab87134) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466)SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-MDM2 antibody [2A10] - ab16895 from Abcam

Prices $403.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2A10
ab16895 staining MDM2 in MCF7 cells treated with dienogest (ab141282), by ICC/IF. Decrease in MDM2 expression correlates with increased concentration of dienogest, as described in literature.The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab141282 (dienogest) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab16895 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-MDM2 antibody [HDM2-323] - ab10567 from Abcam

Prices $372.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone HDM2-323

Anti-MDM2 antibody [SMP 14] - ab3110 from Abcam

Prices $400.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone SMP 14
IHC image of MDM2 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3110, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-MDM2 Antibody (aa26-169) - LS-C25501 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Search more