Anti-MRP1 antibody [MRPr1]

• Supplier: Abcam
• Catalog: ab3368
• Prices: $384.00
• Sizes: 250 µl
• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: MRPr1
• Applications: IHC-P WB IHC-F ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: Bacterial fusion protein of human MRP 1 containing a segment of 168 amino acids inthe amino-proximal half of the protein
• Description: Rat Monoclonal
• Gene: ABCC1
• Conjugate: Unconjugated

Product images

ICC/IF image of ab3368 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3368,5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC image of ab3368 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3368, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a - Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a

Hirata N, Yamada S, Shoda T, Kurihara M, Sekino Y, Kanda Y. Nat Commun. 2014 Sep 25;5:4806.

Efflux protein expression in human stem cell-derived retinal pigment epithelial - Efflux protein expression in human stem cell-derived retinal pigment epithelial

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Inhibition of MRP4 prevents and reverses pulmonary hypertension in mice. - Inhibition of MRP4 prevents and reverses pulmonary hypertension in mice.

Hara Y, Sassi Y, Guibert C, Gambaryan N, Dorfmuller P, Eddahibi S, Lompre AM, Humbert M, Hulot JS. J Clin Invest. 2011 Jul;121(7):2888-97.

Efflux protein expression in human retinal pigment epithelium cell lines. - Efflux protein expression in human retinal pigment epithelium cell lines.

Mannermaa E, Vellonen KS, Ryhanen T, Kokkonen K, Ranta VP, Kaarniranta K, Urtti A. Pharm Res. 2009 Jul;26(7):1785-91.