Anti-N Cadherin antibody [5D5]
| Name | Anti-N Cadherin antibody [5D5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab98952 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 5D5 |
| Applications | FC IHC-F WB IHC-P ICC/IF ICC/IF ELISA |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Purified recombinant fragment of Human N Cadherin expressed in E |
| Description | Mouse Monoclonal |
| Gene | CDH2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-N Cadherin antibody [5D5] (ab98952) at 1/500 dilutionLane 1 : A431 cell lysateLane 2 : NIH-3T3 cell lysateLane 3 : HeLa cell lysateLane 4 : C6 cell lysateLane 5 : LNCap cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27141_N-Cadherin-Primary-antibodies-ab98952-1.jpg)
All lanes : Anti-N Cadherin antibody [5D5] (ab98952) at 1/500 dilutionLane 1 : A431 cell lysateLane 2 : NIH-3T3 cell lysateLane 3 : HeLa cell lysateLane 4 : C6 cell lysateLane 5 : LNCap cell lysate
ab98952 staining N Cadherin in paraffin-embedded human lung cancer tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with HOPE and blocked. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A TRITC-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.See Abreview
Immunofluorescence analysis of A431 cells using ab98952 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye
ab98952 staining N Cadherin in human NSCLC tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with using HOPE technique and blocked for 5 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.See Abreview
Immunohistochemical analysis of paraffin-embedded Human lung cancer (1), colon cancer (2), ovarian cancer (3) and mammary cancer (4), using ab98952 at 1/200 dilution with DAB staining.
ab98952 staining N Cadherin (orange) in Mouse E15.5 Brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBST) for 12 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.See Abreview
![Overlay histogram showing HeLa cells stained with ab98952 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab98952, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/27147_N-Cadherin-Primary-antibodies-ab98952-15.jpg)
Overlay histogram showing HeLa cells stained with ab98952 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab98952, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
FKBP51 increases the tumour-promoter potential of TGF-beta. - FKBP51 increases the tumour-promoter potential of TGF-beta.
Romano S, D'Angelillo A, D'Arrigo P, Staibano S, Greco A, Brunetti A, Scalvenzi M, Bisogni R, Scala I, Romano MF. Clin Transl Med. 2014 Jan 27;3(1):1.
Btbd7 contributes to reduced E-cadherin expression and predicts poor prognosis in - Btbd7 contributes to reduced E-cadherin expression and predicts poor prognosis in
Fan C, Miao Y, Zhang X, Liu D, Jiang G, Lin X, Han Q, Luan L, Xu Z, Wang E. BMC Cancer. 2014 Sep 24;14:704.