Anti-NFkB Inducing Kinase NIK antibody

• Supplier: Abcam
• Catalog: ab7204
• Prices: $392.00
• Sizes: 50 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IHC-P WB IP
• Species Reactivities: Human
• Antigen: Recombinant fragment corresponding to Human NFkB Inducing Kinase NIK aa 795-947
• Description: Rabbit Polyclonal
• Gene: MAP3K14
• Conjugate: Unconjugated

Product images

All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab7204)Lane 1 : macrophages lysateLane 2 : NIK transfected cell lysate

ICC/IF image of ab7204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Downregulation of TRAF2 mediates NIK-induced pancreatic cancer cell proliferation - Downregulation of TRAF2 mediates NIK-induced pancreatic cancer cell proliferation

Doppler H, Liou GY, Storz P. PLoS One. 2013;8(1):e53676.

Hodgkin lymphoma requires stabilized NIK and constitutive RelB expression for - Hodgkin lymphoma requires stabilized NIK and constitutive RelB expression for

Ranuncolo SM, Pittaluga S, Evbuomwan MO, Jaffe ES, Lewis BA. Blood. 2012 Nov 1;120(18):3756-63.

A diverse range of gene products are effectors of the type I interferon antiviral - A diverse range of gene products are effectors of the type I interferon antiviral

Schoggins JW, Wilson SJ, Panis M, Murphy MY, Jones CT, Bieniasz P, Rice CM. Nature. 2011 Apr 28;472(7344):481-5.