ab104535 at 1/1000 dilution staining NG2 in Human breast cancer by Immunohistochemistry, Paraffin-embedded tissue.
ab104535 at 1/1000 dilution staining NG2 in Human breast cancer by Immunohistochemistry, Paraffin-embedded tissue.
ab104535 at 1/1000 dilution staining NG2 in Human breast cancer by Immunohistochemistry, Paraffin-embedded tissue.
ab104535 at 1/1000 dilution staining NG2 in Human breast cancer by Immunohistochemistry, Paraffin-embedded tissue.
ab104535 at 1/1000 dilution staining NG2 in Human breast cancer by Immunohistochemistry, Paraffin-embedded tissue.
ab104535 at 1/1000 dilution staining NG2 in Human breast cancer by Immunohistochemistry, Paraffin-embedded tissue.
ICC/IF image of ab104535 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab104535, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.