CSPG4

Gene Symbol CSPG4
Entrez Gene 1464
Alt Symbol HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2
Species Human
Gene Type protein-coding
Description chondroitin sulfate proteoglycan 4
Other Description chondroitin sulfate proteoglycan 4 (melanoma-associated)|chondroitin sulfate proteoglycan NG2|melanoma chondroitin sulfate proteoglycan|melanoma-associated chondroitin sulfate proteoglycan
Swissprots Q6UVK1 Q92675 D3DW77
Accessions EAW99239 EAW99240 Q6UVK1 AY359468 AAQ62842 BC033194 BC128110 AAI28111 BC172270 AAI72270 BC172576 AAI72576 BP363063 BQ679485 CA428796 CA438367 CB044012 X96753 CAA65529 NM_001897 NP_001888
Function Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activat
Subcellular Location Apical cell membrane {ECO:0000250}; Single- pass type I membrane protein {ECO:0000250}; Extracellular side {ECO:0000250}. Cell projection, lamellipodium membrane {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}; Extracellular side {ECO:0000250}. Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity). {ECO:0000250}.
Tissue Specificity Detected only in malignant melanoma cells. {ECO:0000269|PubMed:8790396}.

NG2 Antibody - 4235 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MDA-MB-468 and RN33B cells using NG2 Antibody.

NG2 (D-20) - sc-30922 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NG2 (F-12) - sc-166179 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NG2 (F-12): sc-166179. Western blot analysis of NG2 expression in SK-MEL-24 whole cell lysate.

NG2 (G-20) - sc-30923 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NG2 (G-20): sc-30923. Western blot analysis of human recombinant NG2 fusion protein.

NG2 (L-20) - sc-30921 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NG2 (H-300) - sc-20162 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NG2 (H-300): sc-20162. Western blot analysis of NG2 expression in SK-MEL-28 whole cell lysate.

NG2 (G-9) - sc-166251 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NG2 (G-9): sc-166251. Western blot analysis of NG2 expression in SK-MEL-24 whole cell lysate.

NG2 (7.1) - sc-53508 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

NG2 (LHM 2) - sc-53389 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NG2 (LHM 2): sc-53389. Western blot analysis of NG2 expression in SK-MEL-24 whole cell lysate.

NG2 (9.2.27) - sc-80003 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

p-NG2 (Thr 2252) - sc-33038 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-NG2 antibody - ab83508 from Abcam

Prices $392.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labelling NG2 with ab83508 at 1/50. 4μm-thick sections were deparaffinized in xylene, dehydrated through three alcohol changes. Endogenous peroxidase activity was quenched with 3% hydrogen peroxide in methanol. Antigen retrieval was performed in 96°C solution of 0.01 mol/L sodium citrate buffer (pH 6.0) for 30 minutes. Peroxidase/DAB, Rabbit/Mouse kit, after visualization, the sections were counterstained with hematoxylin. Hisg NG2 expression.

Anti-NG2 antibody - ab83178 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC-FoFr image of NG2 staining on sections of rat cortex post injury using ab83178 (1:100). The brain was perfusion fixed using 4% PFA and the sections were permeabilized using 0.1% TritonX in 0.1% PBS. The sections were then blocked using 10% donkey serum for 1hour at 24°C. ab83178 was diluted 1:100 and incubated with the sections for 24 hours at 4°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000).

Anti-NG2 antibody - ab104535 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab104535 at 1/1000 dilution staining NG2 in Human breast cancer by Immunohistochemistry, Paraffin-embedded tissue.

Anti-NG2 antibody - ab129051 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab129051 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129051 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NG2 antibody - ab86067 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NG2 antibody (ab86067) at 0.04 µg/mlLane 1 : Hela whole cell lysate at 50 µgLane 2 : Hela whole cell lysate at 15 µgLane 3 : Hela whole cell lysate at 5 µgdeveloped using the ECL technique

Anti-NG2 antibody [ EPR9195] - ab139406 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9195
Anti-NG2 antibody [ EPR9195] (ab139406) at 1/1000 dilution + A375 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-NG2 antibody [LHM 2] - ab20156 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone LHM 2

Anti-CSPG / NG2 Antibody - LS-C79283 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-CSPG / NG2 Antibody - LS-C152910 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-CSPG4 / NG2 Antibody - LS-C22107 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-CSPG4 / NG2 Antibody - LS-C22108 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-CSPG4 / NG2 Antibody (clone 9.9.27) - LS-C22105 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 9.9.27

Anti-CSPG4 / NG2 Antibody - LS-C22112 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-CSPG4 / NG2 Antibody - LS-C22106 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
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