Anti-NPHS2 antibody
| Name | Anti-NPHS2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab50339 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: SKPVEPLNPKKKDSPML conjugated to KLH by an N-terminal Cysteine residue linker, corresponding to amino acids 367-383 of Human NPHS2 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | NPHS2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab50339 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50339, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Lutheran/basal cell adhesion molecule accelerates progression of crescentic - Lutheran/basal cell adhesion molecule accelerates progression of crescentic
Huang J, Filipe A, Rahuel C, Bonnin P, Mesnard L, Guerin C, Wang Y, Le Van Kim C, Colin Y, Tharaux PL. Kidney Int. 2014 May;85(5):1123-36.
Cell-specific translational profiling in acute kidney injury. - Cell-specific translational profiling in acute kidney injury.
Liu J, Krautzberger AM, Sui SH, Hofmann OM, Chen Y, Baetscher M, Grgic I, Kumar S, Humphreys BD, Hide WA, McMahon AP. J Clin Invest. 2014 Mar;124(3):1242-54.
Heterogeneous genetic alterations in sporadic nephrotic syndrome associate with - Heterogeneous genetic alterations in sporadic nephrotic syndrome associate with
Giglio S, Provenzano A, Mazzinghi B, Becherucci F, Giunti L, Sansavini G, Ravaglia F, Roperto RM, Farsetti S, Benetti E, Rotondi M, Murer L, Lazzeri E, Lasagni L, Materassi M, Romagnani P. J Am Soc Nephrol. 2015 Jan;26(1):230-6.
Regeneration and experimental orthotopic transplantation of a bioengineered - Regeneration and experimental orthotopic transplantation of a bioengineered
Song JJ, Guyette JP, Gilpin SE, Gonzalez G, Vacanti JP, Ott HC. Nat Med. 2013 May;19(5):646-51.
Nicorandil as a novel therapy for advanced diabetic nephropathy in the - Nicorandil as a novel therapy for advanced diabetic nephropathy in the
Tanabe K, Lanaspa MA, Kitagawa W, Rivard CJ, Miyazaki M, Klawitter J, Schreiner GF, Saleem MA, Mathieson PW, Makino H, Johnson RJ, Nakagawa T. Am J Physiol Renal Physiol. 2012 May 1;302(9):F1151-60. doi:
The PI3K/Akt/mTOR pathway is activated in murine lupus nephritis and - The PI3K/Akt/mTOR pathway is activated in murine lupus nephritis and
Stylianou K, Petrakis I, Mavroeidi V, Stratakis S, Vardaki E, Perakis K, Stratigis S, Passam A, Papadogiorgaki E, Giannakakis K, Nakopoulou L, Daphnis E. Nephrol Dial Transplant. 2011 Feb;26(2):498-508.
Role of smooth muscle protein SM22alpha in glomerular epithelial cell injury. - Role of smooth muscle protein SM22alpha in glomerular epithelial cell injury.
Marshall CB, Krofft RD, Blonski MJ, Kowalewska J, Logar CM, Pippin JW, Kim F, Feil R, Alpers CE, Shankland SJ. Am J Physiol Renal Physiol. 2011 Apr;300(4):F1026-42. doi:
Podocyte main slit diaphragm proteins, nephrin and podocin, are affected at early - Podocyte main slit diaphragm proteins, nephrin and podocin, are affected at early
Perysinaki GS, Moysiadis DK, Bertsias G, Giannopoulou I, Kyriacou K, Nakopoulou L, Boumpas DT, Daphnis E. Lupus. 2011 Jul;20(8):781-91.
Low-level C-reactive protein levels exert cytoprotective actions on human - Low-level C-reactive protein levels exert cytoprotective actions on human
Pawluczyk IZ, Yang B, Patel SR, Saleem MA, Topham PS. Nephrol Dial Transplant. 2011 Aug;26(8):2465-75.
Inhibition of estradiol synthesis attenuates renal injury in male - Inhibition of estradiol synthesis attenuates renal injury in male
Manigrasso MB, Sawyer RT, Marbury DC, Flynn ER, Maric C. Am J Physiol Renal Physiol. 2011 Sep;301(3):F634-40. doi: