NPHS2

Gene Symbol NPHS2
Entrez Gene 7827
Alt Symbol PDCN, SRN1
Species Human
Gene Type protein-coding
Description nephrosis 2, idiopathic, steroid-resistant (podocin)
Other Description podocin
Swissprots Q8N6Q5 B1AM32 B1AM33 Q9NP85
Accessions CAB83272 EAW91049 EAW91050 Q9NP85 AJ279254 CAB83216 BC029141 AAH29141 BI831348 BX095913 XM_005245483 XP_005245540 XM_006711529 XP_006711592 NM_001297575 NP_001284504 NM_014625 NP_055440
Function Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.
Subcellular Location Isoform 1: Cell membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
Tissue Specificity Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.

Podocin (H-130) - sc-21009 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Podocin (H-130): sc-21009. Western blot analysis of Podocin expression in TE671 (A) and Caki-1 (B) whole cell lysates and rat cerebellum (C), mouse cerebellum (D) and mouse kidney (E) tissue extracts.

Podocin (N-21) - sc-22294 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Podocin (C-18) - sc-22296 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-NPHS2 antibody - ab50339 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab50339 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50339, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-NPHS2 antibody - ab93650 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of NPHS2 staining in THuman normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93650, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-NPHS2 antibody - ab168625 from Abcam

Prices $349.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-NPHS2 antibody (ab168625) at 1 µg/ml + Human placenta tissue lysate at 50 µgdeveloped using the ECL technique

Anti-NPHS2 antibody [EPR13819] - ab183703 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13819
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NPHS2 with ab183703 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Anti-NPHS2 antibody [EPR13820] - ab181143 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13820
Anti-NPHS2 antibody [EPR13820] (ab181143) at 1/50000 dilution + Human fetal kidney lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Anti-NPHS2 antibody, prediluted - ab82107 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human kidney tissue using prediluted ab82107.

Anti-NPHS2 antibody - N-terminal - ab94995 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NPHS2 antibody - N-terminal (ab94995) at 1/100 dilution + HepG2 cell line lysates at 35 µg

Podocin/NPHS2 Antibody - NBP2-33570 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: Podocin/NPHS2 Antibody [NBP2-33570] - NPHS2 Antibody [NBP2-33570] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.

Podocin/NPHS2 Antibody - H00007827-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: NPHS2 Antibody [H00007827-D01P] - Analysis of NPHS2 expression in mouse kidney.

Podocin/NPHS2 Antibody - NBP2-26057 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: NPHS2 Antibody [NBP2-26057] - staining of Human (A) and Mouse (B) Kidney lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NPHS2 Antibody - H00007827-D01 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Podocin/NPHS2 Antibody - H00007827-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: Podocin/NPHS2 Antibody [H00007827-B01P] - Analysis of NPHS2 expression in human placenta.

Podocin Polyclonal Antibody - bs-6597R from Bioss

Prices $279.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded labeled with Rabbit Anti-Podocin Polyclonal Antibody, Unconjugated (bs-6597R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Anti-NPHS2 antibody produced in rabbit - HPA049486 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NPHS2: <B>Cat. No. HPA049486</B>: Immunohistochemical staining of Human kidney shows strong membranous positivity in cells in glomeruli.

Anti-NPHS2 antibody produced in rabbit - SAB1411415 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-NPHS2: <B>Cat. No. SAB1411415</B>: Antibody concentration: 1-5 μg/mL. Specific band of ~34.4 kDa using mouse kidney lysate.

Anti-NPHS2 antibody produced in mouse - SAB1406635 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-NPHS2: <B>Cat. No. SAB1406635</B>: Antibody concentration: 1-5 μg/mL. Specific band of ~34.4 kDa using human placenta lysate.

Anti-NPHS2 antibody produced in rabbit - AV47047 from Sigma-Aldrich

Prices $347.50
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: fetal liver (<B>Cat. No. AV47047</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1 μg/mL in cell type fetal liver

NPHS2--SRN1-Antibody-C-Term - AF3998a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF3998a (0.3 µg/ml) staining of Human (A) and Mouse (B) Kidney lysates (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Podocin Antibody (OAAF05922) - OAAF05922 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

NPHS2 antibody - orb77373 from Biorbyt

Prices $425.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Western blot analysis of fetal kidney lysates using NPHS2 antibody

NPHS2 antibody - orb101513 from Biorbyt

Prices $255.00, $340.00
Sizes 100 μg, 200 μg
Host Rabbit
Clonality Polyclonal
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of NPHS2 antibody

Podocin Polyclonal Antibody (OABB00281) - OABB00281 from Aviva Systems Biology

Prices $255.00
Sizes 100 µg
Clonality Polyclonal
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