Anti-p27 KIP 1 (phospho T187) antibody
| Name | Anti-p27 KIP 1 (phospho T187) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab75908 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic phosphopeptide derived from human p27 KIP 1 around the phosphorylation site of threonine 187 (E-Q-TP-P-K) |
| Description | Rabbit Polyclonal |
| Gene | CDKN1B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab75908 stained A431 cells (ab7909). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75908, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-p27 KIP 1 (phospho T187) antibody (ab75908) at 1/500 dilutionLane 1 : Extracts from HeLa cells treated with EGF with immunising peptideLane 2 : Extracts from HeLa cells treated with EGFLane 3 : Extracts from HeLa cells treated with IFN alpha
Product References
MAPK signaling pathway regulates p27 phosphorylation at threonin 187 as part of - MAPK signaling pathway regulates p27 phosphorylation at threonin 187 as part of
Osaki LH, Gama P. PLoS One. 2013 Jun 7;8(6):e66651.
Targeted deletion of Capn4 in cells of the chondrocyte lineage impairs - Targeted deletion of Capn4 in cells of the chondrocyte lineage impairs
Kashiwagi A, Schipani E, Fein MJ, Greer PA, Shimada M. Mol Cell Biol. 2010 Jun;30(11):2799-810.