CDKN1B

Gene Symbol CDKN1B
Entrez Gene 1027
Alt Symbol CDKN4, KIP1, MEN1B, MEN4, P27KIP1
Species Human
Gene Type protein-coding
Description cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Other Description cyclin-dependent kinase inhibitor 1B
Swissprots Q9BUS6 Q5U0H2 P46527 Q16307
Accessions AAD14244 AAF21058 AAL78041 ABS17636 BAA76715 BAA88240 CAA59284 CAN37616 CBX53898 EAW96275 P46527 AA833962 AB451291 BAG70105 AB451423 BAG70237 AF247551 AAF69497 AJ616234 CAE82383 AK298335 BAG60584 AK312461 BAG35368 AM392564 CAL37442 AM393540 CAL38417 AM393634 CAL38510 AM393862 CAL38737 AY004255 AAF88142 BC001971 AAH01971 BG701047 BT019553 AAV38360 BT019554 AAV38361 CN311141 CR457399 CAG33680 DA948043 HY097069 HY246392 U10906 AAA20240 NM_004064 NP_004055
Function Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry. {ECO:0000269|PubMed:10831586, ECO:0000269|PubMed:12244301, ECO:0000269|PubMed:16782892, ECO:0000269|PubMed:17254966, ECO:0000269|PubMed:19075005}.
Subcellular Location Nucleus. Cytoplasm. Endosome {ECO:0000250}. Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity). {ECO:0000250}.
Tissue Specificity Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
Top Pathways Viral carcinogenesis, Epstein-Barr virus infection, MicroRNAs in cancer, PI3K-Akt signaling pathway, Cell cycle

p27 Kip1 (D69C12) XP ® Rabbit mAb (PE Conjugate) - 12184 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D69C12
Flow cytometric analysis of Jurkat cells using p27 Kip1 (D69C12) XP ® Rabbit mAb (PE Conjugate) and DRAQ5 ® #4084 (fluorescent DNA dye).

p27 Kip1 (D69C12) XP ® Rabbit mAb (Biotinylated) - 13715 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D69C12
Western blot analysis of extracts from COS and MCF7 cells using p27 Kip1 (D69C12) XP ® Rabbit mAb (Biotinylated).

p27 Kip1 (D69C12) XP ® Rabbit mAb - 3686 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D69C12
Western blot analysis of extracts from various cell types using p27 Kip1 (D69C12) XP ® Rabbit mAb.

p27 Kip1 Antibody - 2552 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from C6, NIH/3T3, HeLa and MCF-7 cells, using p27 Kip1 Antibody.

p27 Kip1 (SX53G8.5) Mouse mAb - 3698 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone SX53G8.5
Western blot analysis of extracts from NIH/3T3, C6 and MCF7 cells using p27 Kip1 (SX53G8.5) Mouse mAb.

p27 Kip1 (D37H1) Rabbit mAb - 3688 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D37H1
Western blot analysis of extracts from various cell types using p27 Kip1 (D37H1) Rabbit mAb.

p27 (A-10) - sc-393380 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p27 (A-10): sc-393380. Western blot analysis of p27 expression in non-transfected: sc-117752 (A) and human p27 transfected: sc-110470 (B) 293T whole cell lysates.

p27 (C-19) - sc-528 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p27 (C-19)-G: sc-528-G. Western blot analysis of His-tagged mouse recombinant p27.

p27 (F-8) - sc-1641 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p27 (F-8): sc-1641. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization of p27.

p27 (D-2) - sc-393305 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p27 (D-2): sc-393305. Western blot analysis of p27 expression in non-transfected: sc-110760 (A) and human p27 transfected: sc-110470 (B) 293 whole cell lysates.

p27 (M-197) - sc-776 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of p27 expression in Jurkat (A) and NIH/3T3 (B) whole cell lysates. Antibodies used include: p27 (M-197): sc-776 (A) and p27 (N-20): sc-527 (B).

p27 (N-20) - sc-527 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p27 (N-20)-G: sc-527-G. Western blot analysis of human recombinant p27 fusion protein.

p27 (SX18F7) - sc-53906 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p27 (SX18F7): sc-53906. Western blot analysis of p27 expression in Jurkat (A), HeLa (B), MCF7 (C) and BT-20 (D) whole cell lysates.

p27 (SX53G8.5) - sc-53871 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p27 (SX53G8.5): sc-53871. Western blot analysis of p27 expression in non-transfected: sc-110760 (A) and human p27 transfected: sc-110470 (B) 293 whole cell lysates.

p27 (SPM348) - sc-56454 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
p27 (SPM348): sc-56454. Western blot analysis of p27 expression in Jurkat (A), HeLa (B) and MCF7 (C) whole cell lysates.

p-p27 (Thr 187) - sc-16324 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-p27 (Thr 187)-R: sc-16324-R. Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse skeletal muscle showing cytoskeletal localization.

p-p27 (Thr 198) - sc-130603 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-p27 KIP 1 (phospho S10) antibody - ab36727 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-p27 KIP 1 (phospho S10) antibody [EP233(2)Y] - ab62364 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP233(2)Y
Immunohistochemistical detection of p27 KIP 1 (phospho S10) antibody [EP233(2)Y] (ab62364) on formaldehyde fixed paraffin-embedded monkey cerebellum sections. Antigen retrieval step: Heat mediated. Buffer: Citric acid solution. Permeabilization: None. Blocking step: 1% BSA for 10 mins. Primary antibody incubated at 1/1500 for 2 hours @ 21°C in TBS/BSA/azide. Secondary antibody Name: anti rabbit IgG conjugated to biotin (1/200).See Abreview

Anti-p27 KIP 1 (phospho T157) antibody - ab60019 from Abcam

Prices $304.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-p27 KIP 1 (phospho T157) antibody (ab60019) at 1/100 dilution + HL60 tissue lysate

Anti-p27 KIP 1 (phospho T157) antibody - ab111614 from Abcam

Host Rabbit
Clonality Polyclonal
ab111614 at 1/50 dilution staining p27 KIP 1 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

Anti-p27 KIP 1 (phospho T187) antibody - ab75908 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab75908 stained A431 cells (ab7909). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75908, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-p27 KIP 1 (phospho T187) antibody - ab118644 from Abcam

Host Rabbit
Clonality Polyclonal
ab118644 at 5 µg/ml staining p27 KIP 1 in Formalin-Fixed, Paraffin-Embedded Human Intestine tissue by Immunohistochemistry.

Anti-p27 KIP 1 (phospho T187) antibody - ab138661 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-p27 KIP 1 (phospho T187) antibody (ab138661) at 1/500 dilution + extracts from HeLa cells treated with EGF or IFN alpha at 30 µg

Anti-p27 KIP 1 (phospho T198) antibody - ab64949 from Abcam

Prices $388.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-p27 KIP 1 (phospho T198) antibody (ab64949) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : HepG2 cell extractLane 3 : COLO205 cell extractLane 4 : COLO205 cell extract with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.
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