![PAX5 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to PAX5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15164.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 42kDa; PAX5](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/7704_ab15164-209878-IPVI004ab1516420m.jpg)
PAX5 was immunoprecipitated using 0.5mg Ramos whole cell extract, 5µg of Rabbit polyclonal to PAX5 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Ramos whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab15164.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 42kDa; PAX5
ab15164 staining PAX5 in human tonsil by Immunohistochemistry (FFPE-sections).