Anti-Peroxiredoxin 1 antibody [EPR5433]
| Name | Anti-Peroxiredoxin 1 antibody [EPR5433] |
|---|---|
| Supplier | Abcam |
| Catalog | ab109498 |
| Prices | $388.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR5433 |
| Applications | WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | A synthetic peptide corresponding to residues in Human Peroxiredoxin 1 |
| Description | Rabbit Monoclonal |
| Gene | PRDX1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HeLa cells stained with ab109498 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109498, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-Peroxiredoxin 1 antibody [EPR5433] (ab109498) at 1/10000 dilutionLane 1 : MCF-7 cell lysateLane 2 : 293T cell lysateLane 3 : K562 cell lysates Lysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/10008_Peroxiredoxin-1-Primary-antibodies-ab109498-7.jpg)
All lanes : Anti-Peroxiredoxin 1 antibody [EPR5433] (ab109498) at 1/10000 dilutionLane 1 : MCF-7 cell lysateLane 2 : 293T cell lysateLane 3 : K562 cell lysates Lysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded kidney tissue using ab109498 at 1/250
Immunohistochemical analysis of paraffin-embedded thyroid carcinoma tissue using ab109498 at 1/250.
Immunofluorescent analysis of 293T cells labeled with ab109498 at 1/10.
Product References
Peroxiredoxin post-translational modifications by redox messengers. - Peroxiredoxin post-translational modifications by redox messengers.
Riquier S, Breton J, Abbas K, Cornu D, Bouton C, Drapier JC. Redox Biol. 2014 Jun 5;2:777-85.
Sodium hydrosulfide inhibits the differentiation of osteoclast progenitor cells - Sodium hydrosulfide inhibits the differentiation of osteoclast progenitor cells
Gambari L, Lisignoli G, Cattini L, Manferdini C, Facchini A, Grassi F. Pharmacol Res. 2014 Sep;87:99-112.