ICC/IF image of ab71090 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab71090 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-PI 3 Kinase catalytic subunit alpha antibody (ab71090) at 1/60 dilution + K562 cell lysate at 35 µg
All lanes : Anti-PI 3 Kinase catalytic subunit alpha antibody (ab71090) at 1/60 dilutionLane 1 : Non transfected 293 cell lysateLane 2 : 293 cell lysate transiently transfected with the PI 3 Kinase catalytic subunit alpha geneLysates/proteins at 1 µg per lane.
Formalin fixed and paraffin embedded human breast carcinoma tissue labelled with ab71090 at a 1/50 dilution. ab71090 was peroxidase conjugated to the secondary antibody, followed by DAB staining.