Anti-PI3 Kinase p110 beta antibody
| Name | Anti-PI3 Kinase p110 beta antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55593 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | IHC-P WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 147-257 of Human PI3 Kinase p110 beta |
| Description | Mouse Monoclonal |
| Gene | PIK3CB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
PI3 Kinase p110 beta antibody (ab55593) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lymph node.
Western blot against tagged recombinant protein immunogen using ab55593 PI3 Kinase p110 beta antibody at 1ug/ml.
![Overlay histogram showing Jurkat cells stained with ab55593 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55593, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/11686_PI3-Kinase-p110-beta-Primary-antibodies-ab55593-1.jpg)
Overlay histogram showing Jurkat cells stained with ab55593 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55593, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Targeting class IA PI3K isoforms selectively impairs cell growth, survival, and - Targeting class IA PI3K isoforms selectively impairs cell growth, survival, and
Holand K, Boller D, Hagel C, Dolski S, Treszl A, Pardo OE, Cwiek P, Salm F, Leni Z, Shepherd PR, Styp-Rekowska B, Djonov V, von Bueren AO, Frei K, Arcaro A. PLoS One. 2014 Apr 9;9(4):e94132.
Targeting the phosphoinositide 3-kinase p110-alpha isoform impairs cell - Targeting the phosphoinositide 3-kinase p110-alpha isoform impairs cell
Wojtalla A, Fischer B, Kotelevets N, Mauri FA, Sobek J, Rehrauer H, Wotzkow C, Tschan MP, Seckl MJ, Zangemeister-Wittke U, Arcaro A. Clin Cancer Res. 2013 Jan 1;19(1):96-105.
PIKing the right isoform: the emergent role of the p110beta subunit in breast - PIKing the right isoform: the emergent role of the p110beta subunit in breast
Carvalho S, Milanezi F, Costa JL, Amendoeira I, Schmitt F. Virchows Arch. 2010 Mar;456(3):235-43.