Anti-PI3 Kinase p110 beta antibody [EPR5515(2)]

• Supplier: Abcam
• Catalog: ab151549
• Prices: $99.00, $387.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR5515(2)
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
• Description: Rabbit Monoclonal
• Gene: PIK3CB
• Conjugate: Unconjugated

Product images

Overlay histogram showing SH-SY5Y cells stained with ab131260 (red line) at 1/20 dilution. The cells were fixed with 2% Paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

ab151549 staining PI3 Kinase p110 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/150).ab150082 an Alexa Fluor®555-conjugated Goat anti-rabbit IgG(1/500) was used as the secondary antibody. Nuclei were counterstained with DAPI.

ab151549 staining PI3 Kinase p110 beta in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/75). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilutionLane 1 : Rat Brain LysateLane 2 : Mouse Brain LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/5000 dilution + K562 Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilutionLane 1 : 293 Cell LysateLane 2 : Jurket Cell LysateLane 3 : MCF-7 Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

Overlay histogram showing HeLa cells stained with unpurified ab151549 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151549, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-PI3 Kinase p110 beta antibody [EPR5515(2)] (ab151549) at 1/1000 dilution (unpurified)Lane 1 : MCF7 cell lysateLane 2 : Jurkat cell lysateLane 3 : K562 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/50 dilution.

Immunofluorescent analysis of 293 cells labelling PI3 Kinase p110 beta with unpurified ab151549 at 1/10 dilution.

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using unpurified ab151549 showing +ve staining.

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using unpurified ab151549 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using unpurified ab151549 showing +ve staining.

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using unpurified ab151549 showing +ve staining.

Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using unpurified ab151549 showing +ve staining.