Anti-PKC beta 2 antibody [Y125]
| Name | Anti-PKC beta 2 antibody [Y125] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32026 |
| Prices | $99.00, $401.00 |
| Sizes | 10 µl, 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | Y125 |
| Applications | WB IHC-P ICC/IF ICC/IF FC IP |
| Species Reactivities | Mouse, Rat, Human, Bovine, Pig |
| Antigen | ab32026 recognises PKC beta 2, it does not cross react with human PKC beta 1 |
| Description | Rabbit Monoclonal |
| Gene | PRKCB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution + K562 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/12527_ab32026_1.JPG)
Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution + K562 cell lysate
ab32026, at a 1/100 dilution, staining human PKC beta 2 in breast carcinoma by Immunohistochemistry, Paraffin embedded tissue
ab32026, at a 1/250 dilution, staining human PKC beta 2 in HeLa cells by Immunoflourescence
Overlay histogram showing HeLa cells stained with ab32026 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32026, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Trimeric G protein-CARMA1 axis links smoothened, the hedgehog receptor - Trimeric G protein-CARMA1 axis links smoothened, the hedgehog receptor
Qu C, Liu Y, Kunkalla K, Singh RR, Blonska M, Lin X, Agarwal NK, Vega F. Blood. 2013 Jun 6;121(23):4718-28.
Conventional protein kinase C-alpha (PKC-alpha) and PKC-beta negatively regulate - Conventional protein kinase C-alpha (PKC-alpha) and PKC-beta negatively regulate
Maharaj NP, Wies E, Stoll A, Gack MU. J Virol. 2012 Feb;86(3):1358-71.
Adaptive basal phosphorylation of eIF2alpha is responsible for resistance to - Adaptive basal phosphorylation of eIF2alpha is responsible for resistance to
Zeng N, Li Y, He L, Xu X, Galicia V, Deng C, Stiles BL. Mol Cancer Res. 2011 Dec;9(12):1708-17.
Differential subcellular expression of protein kinase C betaII in breast cancer: - Differential subcellular expression of protein kinase C betaII in breast cancer:
Gokmen-Polar Y, Mehta R, Tuzmen S, Mousses S, Thorat MA, Sanders KL, Turbin D, Leung S, Huntsman DG, Sledge GW Jr, Badve S. Breast Cancer Res Treat. 2010 Nov;124(2):327-35.