PRKCB

Gene Symbol PRKCB
Entrez Gene 5579
Alt Symbol PKC-beta, PKCB, PRKCB1, PRKCB2
Species Human
Gene Type protein-coding
Description protein kinase C, beta
Other Description PKC-B|protein kinase C beta type|protein kinase C, beta 1 polypeptide
Swissprots Q9UE50 D3DWF5 Q15138 C5IFJ8 O43744 Q9UEH8 P05127 Q9UE49 Q93060 Q9UJ33 Q9UJ30 P05771
Accessions AAA60096 AAA60097 AAB97933 AAB97934 AAD13852 ACS14045 BAA00912 CAA05725 CAA29395 CAA29396 CAA44393 CAZ00742 EAW55796 EAW55797 EAW55798 EAW55799 P05771 AA613106 AI423262 AJ002788 AK057555 AK123381 AL833252 BC036472 AAH36472 BC045175 BI549165 DB456157 EU832791 ACE87802 GQ129253 ACT64387 M13975 AAA60095 X06318 CAA29634 X07109 CAA30130 NM_002738 NP_002729 NM_212535 NP_997700
Function Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000269|PubMed:20228790}. Membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
Top Pathways Wnt signaling pathway, Tight junction, VEGF signaling pathway, Focal adhesion, Gap junction

PKC βI (C-16) - sc-209 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKC βI (C-16): sc-209. Western blot analysis of PKC βI expression in NIH/3T3 (A), 3611-RF (B), A-431 (C), HeLa (D) and Jurkat (E) whole cell lysates.

PKC βI (E-3) - sc-8049 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC βI (E-3): sc-8049. Western blot analysis of PKC βI expression in 3611-RF whole cell lysate.

PKC βII (C-18) - sc-210 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKC βII (C-18)-G: sc-210-G. Western blot analysis of PKC βII expression in K-562 whole cell lysate.

PKC βII (F-7) - sc-13149 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC βII (F-7): sc-13149. Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with PKC βII (F-7), followed by FITC-conjugated goat anti-mouse IgG: sc-2010. Black line histogram represents the isotype control, normal mouse IgGβ: sc-3877.

p-PKC βI (Thr 641) - sc-101776 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-PKC βI (Thr 641): sc-101776. Western blot analysis of phosphorylated PKC βI expression in untreated (A) and PMA-treated (B) K-562 whole cell lysates.

p-PKC βII/δ (Ser 660) - sc-11760 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot analysis of PKC βIl/δ phosphorylation expression in U-937 cell lysate (A,B). Blots were probed with p-PKC βIl/δ (Ser 660)-R: sc-11760-R preincubated with cognate phosphorylated (A) and cognate non-phosphorylated (B) peptide.

Anti-PKC beta 1 + PKC beta 2 (phospho T500) antibody - ab5817 from Abcam

Prices $380.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5817 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that only the peptide corresponding to PKC beta 1 & 2 [pT500] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

Anti-PKC beta 1 + PKC beta 2 (phospho T641) antibody - ab194749 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC beta 1 + PKC beta 2 (phospho T641) antibody (ab194749) at 1/500 dilutionLane 1 : Extract from jurkat cellsLane 2 : Extract from jurkat cells treated with PMA

Anti-PKC beta 1 + PKC beta 2 antibody - ab5627 from Abcam

Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of PKC beta 1 (green) showing staining in the nucleus and cytoplasm of SW480 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5627 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Anti-PKC beta 1 + PKC beta 2 antibody - ab71123 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PKC beta 1 + PKC beta 2 antibody (ab71123) at 1/100 dilution + human placenta lysate at 12.5 µgSecondaryHRP-anti-rabbit

Anti-PKC beta 1 + PKC beta 2 antibody - N-terminal - ab189782 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC beta 1 + PKC beta 2 antibody - N-terminal (ab189782) at 1/500 dilutionLane 1 : HL60 cell line extractLane 2 : THP1 cell line extractLane 3 : U937 cell line extractLane 4 : HepG2 cell line extractLane 5 : Mouse brain cell line extract

Anti-PKC beta 1 (phospho S661) antibody - ab79307 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PKC beta 1 (phospho S661) antibody - ab192184 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC beta 1 (phospho S661) antibody (ab192184)Lane 1 : Heatshock treated HeLa whole cell lysate with Antigen-specific peptideLane 2 : Heatshock treated HeLa whole cell lysate

Anti-PKC beta 1 (phospho T642) antibody - ab75657 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC beta 1 (phospho T642) antibody (ab75657) at 1/500 dilutionLane 1 : K562 cells, untreatedLane 2 : K562 cells treated with PMA (1ng/ml, 10min)

Anti-PKC beta 1 (phospho T642) antibody - ab5782 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Peptide Competition and Phosphatase Treatment: Lysates prepared from K562 cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda (ë) phosphatase (5), blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab5782 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 5), the non phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that only the peptide corresponding to PKC beta I [pT642] blocks the antibody signal. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

Anti-PKC beta 1 antibody - ab4132 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PKC beta 1 antibody (ab4132) at 1/1000 dilution + Active human PKC beta 1 full length protein (ab60840) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute

Anti-PKC beta 1 antibody - ab137935 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PKC beta 1 antibody (ab137935) at 1/500 dilution + HepG2 cell lysate at 30 µg

Anti-PKC beta 1 antibody [A10-F] - ab136917 from Abcam

Prices $378.00
Sizes 50 µl
Host Rabbit
Clonality Monoclonal
Clone A10-F
Immunofluorescent analysis of HEK293 cells labelling PKC beta 1 with ab136917 at 1/150 dilution.

Anti-PKC beta 1 antibody - C-terminal - ab175446 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling PKC beta 1 with ab175446 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-PKC beta 1 antibody - N-terminal - ab172919 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PKC beta 1 antibody - N-terminal (ab172919) at 1/100 dilution + Mouse brain tissue lysate at 35 µg

Anti-PKC beta 2 (phospho S660) antibody [EP1902Y] - ab75837 from Abcam

Prices $99.00, $385.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1902Y
All lanes : Anti-PKC beta 2 (phospho S660) antibody [EP1902Y] (ab75837) at 1/100000 dilutionLane 1 : Jurkat cell lysates, untreatedLane 2 : Jurkat cell lysates, treated with APLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-PKC beta 2 antibody - ab4138 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PKC beta 2 antibody - ab4136 from Abcam

Prices $370.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-PKC beta 2 antibody [Y125] - ab32026 from Abcam

Prices $99.00, $401.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y125
Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/500 dilution + K562 cell lysate

Anti-PKC beta 2 antibody [Y125] (Alexa Fluor® 488) - ab195251 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone Y125
Overlay histogram showing HeLa cells stained with ab195251 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195251, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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