Anti-PML Protein antibody
| Name | Anti-PML Protein antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53773 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF IP |
| Species Reactivities | Human, Monkey |
| Antigen | Synthetic peptide corresponding to Human PML Protein aa 39-53 (N terminal) |
| Description | Rabbit Polyclonal |
| Gene | PML |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab53773 (1/200) staining PML protein in Hela cells (green). Cells were fixed with methanol, permeabilised with triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.See Abreview
Chromatin was prepared from modified murine D3 ES cells (an in vitro model of RB1 imprinted expression was generated by integrating human PPP1R26P1 into intron 2 of mouse Rb1 in murine embryonic stem cells (mESCs)). Cells were fixed with formaldehyde for 10 mins. Chromatin was sheared into fragment sizes ranging from 200 to 600 bp. For immunoprecipitation 50 μl of sheared chromatin was set aside as input and 20-40 μg of sheared chromatin in a volume of 100 μl was used for antibody incubation. 4 µg of ab53773 to PML was used as a non-related control (black bars) and 4 µl of a rabbit polyclonal to RNAPII CTD repeat YSPTSPS (phospho S5) (shaded bars) was added as the test. The immunoprecipitated DNA was quantified at 3 positions on the the retinoblastoma (Rb1) promoter. 1,2 = Rb1_48; 3,4 = Rb1_69 and 5,6 = Rb1_74
All lanes : Anti-PML Protein antibody (ab53773) at 1/500 dilutionLane 1 : Extracts from A549 cellsLane 2 : Extracts from A549 cells, plus immunising peptide
Ab53773 staining Human normal colon. Staining is localized to the nucleus.Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Product References
Association of BLM and BRCA1 during Telomere Maintenance in ALT Cells. - Association of BLM and BRCA1 during Telomere Maintenance in ALT Cells.
Acharya S, Kaul Z, Gocha AS, Martinez AR, Harris J, Parvin JD, Groden J. PLoS One. 2014 Aug 1;9(8):e103819.
The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin - The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin
Ivanauskiene K, Delbarre E, McGhie JD, Kuntziger T, Wong LH, Collas P. Genome Res. 2014 Oct;24(10):1584-94.
Promyelocytic leukemia (PML) protein plays important roles in regulating cell - Promyelocytic leukemia (PML) protein plays important roles in regulating cell
Tang MK, Liang YJ, Chan JY, Wong SW, Chen E, Yao Y, Gan J, Xiao L, Leung HC, Kung HF, Wang H, Lee KK. PLoS One. 2013;8(3):e59477.
A transmembrane domain and GxxxG motifs within L2 are essential for - A transmembrane domain and GxxxG motifs within L2 are essential for
Bronnimann MP, Chapman JA, Park CK, Campos SK. J Virol. 2013 Jan;87(1):464-73.
DAXX-dependent supply of soluble (H3.3-H4) dimers to PML bodies pending - DAXX-dependent supply of soluble (H3.3-H4) dimers to PML bodies pending
Delbarre E, Ivanauskiene K, Kuntziger T, Collas P. Genome Res. 2013 Mar;23(3):440-51.
Human PPP1R26P1 functions as cis-repressive element in mouse Rb1. - Human PPP1R26P1 functions as cis-repressive element in mouse Rb1.
Steenpass L, Kanber D, Hiber M, Buiting K, Horsthemke B, Lohmann D. PLoS One. 2013 Sep 3;8(9):e74159.
Human sarcomas are mosaic for telomerase-dependent and telomerase-independent - Human sarcomas are mosaic for telomerase-dependent and telomerase-independent
Gocha AR, Nuovo G, Iwenofu OH, Groden J. Am J Pathol. 2013 Jan;182(1):41-8.
FTO levels affect RNA modification and the transcriptome. - FTO levels affect RNA modification and the transcriptome.
Berulava T, Ziehe M, Klein-Hitpass L, Mladenov E, Thomale J, Ruther U, Horsthemke B. Eur J Hum Genet. 2013 Mar;21(3):317-23.
Opposing effects of bacitracin on human papillomavirus type 16 infection: - Opposing effects of bacitracin on human papillomavirus type 16 infection:
Campos SK, Chapman JA, Deymier MJ, Bronnimann MP, Ozbun MA. J Virol. 2012 Apr;86(8):4169-81.
Alternative lengthening of telomeres--an enhanced chromosomal instability in - Alternative lengthening of telomeres--an enhanced chromosomal instability in
Lundberg G, Sehic D, Lansberg JK, Ora I, Frigyesi A, Castel V, Navarro S, Piqueras M, Martinsson T, Noguera R, Gisselsson D. Genes Chromosomes Cancer. 2011 Apr;50(4):250-62.