PML

Gene Symbol PML
Entrez Gene 5371
Alt Symbol MYL, PP8675, RNF71, TRIM19
Species Human
Gene Type protein-coding
Description promyelocytic leukemia
Other Description RING finger protein 71|probable transcription factor PML|promyelocytic leukemia protein|promyelocytic leukemia, inducer of|protein PML|tripartite motif protein TRIM19|tripartite motif-containing protein 19
Swissprots Q00755 Q9BZY2 P29593 E9PBR7 Q8WUA0 Q9BWP7 Q9BZX9 Q96S41 Q15959 Q9BZY0 Q9BZX6 Q9BZX7 P29591 P29592 P29590 Q9BZX8 Q9BPW2 Q59FP9 Q9BZY3
Accessions AAB29490 AAD13865 CAA46026 EAW77946 EAW77947 P29590 AB208950 BAD92187 AB209051 BAD92288 AB209411 BAD92648 AF230401 AAG50180 AF230402 AAG50181 AF230403 AAG50182 AF230404 AAG50183 AF230405 AAG50184 AF230406 AAG50185 AF230407 AAG50186 AF230408 AAG50187 AF230409 AAG50188 AF230410 AAG50189 AF230411 AAG50190 AF370432 AAQ15268 AF388193 AF388194 AK026871 AK096663 AK300256 BAG62021 AK300951 BAG62579 BC000080 AAH00080 BC020994 AAH20994 BC034251 BC139795 AAI39796 BF510860 BT009911 AAP88913 BX647287 EF535849 M73778 AAA60125 M79462 AAA60388 M79463 AAA60351 M79464 AAA60390 M80185 AAA60352 S50913 AAB19601 X63131 CAA44841 NM_002675 NP_002666 NM_033238 NP_150241 NM_033239 NP_150242 NM_033240 NP_150243 NM_033244 NP_150247 NM_033246 NP_150249 NM_033247 NP_150250 NM_033249 NP_150252 NM_033250 NP_150253
Function Functions via its association with PML-nuclear bodies (PML-NBs) in a wide range of important cellular processes, including tumor suppression, transcriptional regulation, apoptosis, senescence, DNA damage response, and viral defense mechanisms. Acts as the scaffold of PML-NBs allowing other proteins to shuttle in and out, a process which is regulated by SUMO-mediated modifications and interactions. Isoform PML-4 has a multifaceted role in the regulation of apoptosis and growth suppression: activates RB1 and inhibits AKT1 via interactions with PP1 and PP2A phosphatases respectively, negatively affects the PI3K pathway by inhibiting MTOR and activating PTEN, and positively regulates p53/TP53 by acting at different levels (by promoting its acetylation and phosphorylation and by inhibiting its MDM2-dependent degradation). Isoform PML-4 also: acts as a transcriptional repressor of TBX2 during cellular senescence and the repression is dependent on a functional RBL2/E2F4 repressor complex, reg
Subcellular Location Nucleus. Nucleus, nucleoplasm. Cytoplasm. Nucleus, PML body. Nucleus, nucleolus. Endoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Note=Isoform PML-1 can shuttle between the nucleus and cytoplasm. Isoform PML-2, isoform PML-3, isoform PML-4, isoform PML-5 and isoform PML-6 are nuclear isoforms whereas isoform PML-7 and isoform PML-14 lacking the nuclear localization signal are cytoplasmic isoforms. Detected in the nucleolus after DNA damage. Acetylation at Lys-487 is essential for its nuclear localization. Within the nucleus, most of PML is expressed in the diffuse nuclear fraction of the nucleoplasm and only a small fraction is found in the matrix- associated nuclear bodies (PML-NBs). The transfer of PML from the nucleoplasm to PML-NBs depends on its phosphorylation and sumoylation. The B1 box and the RING finger are also required for the local
Top Pathways Ubiquitin mediated proteolysis, Acute myeloid leukemia, Endocytosis, Influenza A, Herpes simplex infection

PML (N-19) - sc-9862 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PML (N-19): sc-9862. Western blot analysis of PML expression in mouse cerebellum tissue extract.

PML (E-11) - sc-377390 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PML (E-11): sc-377390. Western blot analysis of PML expression in UV treated HeLa nuclear extract.

PML (PG-M3) - sc-966 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PML (PG-M3): sc-966. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human lymph node showing nuclear staining.

PML (H-238) - sc-5621 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PML (H-238): sc-5621. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.

PML (A-20) - sc-9863 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PML (A-20): sc-9863. Western blot analysis of PML expression in mouse lymph node tissue extract.

PML (C-5) - sc-377303 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PML (C-5): sc-377303. Western blot analysis of PML expression in UV treated HeLa nuclear extracts.

PML (G-8) - sc-377340 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PML (G-8): sc-377340. Western blot analysis of PML expression in K-562 nuclear extract (A) and human lymph node tissue extract (B).

PML (5G191) - sc-71910 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-PML Protein (phospho S518) antibody [EPR6151] - ab128932 from Abcam

Prices $99.00, $313.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6151
All lanes : Anti-PML Protein (phospho S518) antibody [EPR6151] (ab128932) at 1/1000 dilutionLane 1 : HeLa lysate (untreated)Lane 2 : HeLa lysate treated with Alkaline PhosphataseLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-PML Protein antibody - ab53773 from Abcam

Prices $401.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab53773 (1/200) staining PML protein in Hela cells (green). Cells were fixed with methanol, permeabilised with triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.See Abreview

Anti-PML Protein antibody - ab72137 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PML Protein with ab72137 at 1/1000 (0.2µg/ml). Detection: DAB.

Anti-PML Protein antibody - ab31101 from Abcam

Prices $379.00
Sizes 500 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab31101 stained MALME-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31101 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PML Protein antibody - ab80347 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PML Protein antibody (ab80347) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PML Protein antibody - ab57276 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PML Protein antibody - ab94471 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PML Protein antibody (ab94471) at 1 µg/ml (in 5% skim milk / PBS buffer) + ACHN cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PML Protein antibody [C7] - ab96051 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone C7
Ab96051 staining human PML protein in IFNa-treated HL60 cells by immunofluorescence. This image demonstrates the presence of PML nuclear bodies.

Anti-PML Protein antibody [C7] - ab96055 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone C7

Anti-PML Protein antibody [EPR16768] - ab200200 from Abcam

Prices $99.00, $347.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16768
All lanes : Anti-PML Protein antibody [EPR16768] (ab200200) at 1/1000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysateLane 3 : 293 (Human embryonic kidney) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-PML Protein antibody [EPR16792] - ab179466 from Abcam

Prices $99.00, $384.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16792
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling PML Protein with ab179466 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm and nuclear staining on K562 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:--ve control 1 -  ab179466 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

Anti-PML Protein antibody [EPR16792] (Alexa Fluor® 488) - ab200608 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16792
ab200608 staining PML protein in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200608 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-PML Protein antibody [EPR16792] (Alexa Fluor® 647) - ab200521 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16792
ab200521 staining PML in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200521 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-PML Protein antibody [PML-97] - ab50637 from Abcam

Prices $378.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone PML-97

Anti-PML Protein antibody, prediluted - ab31102 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Polyclonal
ab31102 staining human PML in human lymph node tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

Anti-PML+RARA Fusion antibody - ab43152 from Abcam

Prices $242.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
developed using the ECL techniquePerformed under reducing conditions.

Anti-PML Antibody - LS-C331332 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded mouse kidney using PML antibody at dilution of 1:200 (400x lens).
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