Anti-PML Protein antibody

• Supplier: Abcam
• Catalog: ab72137
• Prices: $385.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IP ICC/IF ICC/IF IHC-P
• Species Reactivities: Human, Chimpanzee, Ape, Orangutan
• Antigen: Synthetic peptide corresponding to a region between residues 375 and 425 of human PML Protein, NP_150241
• Description: Rabbit Polyclonal
• Gene: PML
• Conjugate: Unconjugated

Product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PML Protein with ab72137 at 1/1000 (0.2µg/ml). Detection: DAB.

All lanes : Anti-PML Protein antibody (ab72137) at 0.04 µg/mlLane 1 : Whole cell lysate from Hela cells at 50 µgLane 2 : Whole cell lysate from Hela cells at 15 µgLane 3 : Whole cell lysate from Hela cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µg

Immunoprecipitation/ Western Blot of PML Protein. Lane 1: ab72137 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72137 at 1µg/ml for subsequent WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Detection:Chemiluminescence with an exposure time of 3 seconds.

ICC/IF image of ab72137 stained Malme-3M cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72137, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin - The PML-associated protein DEK regulates the balance of H3.3 loading on chromatin

Ivanauskiene K, Delbarre E, McGhie JD, Kuntziger T, Wong LH, Collas P. Genome Res. 2014 Oct;24(10):1584-94.

Kaposi's sarcoma-associated herpesvirus K-Rta exhibits SUMO-targeting ubiquitin - Kaposi's sarcoma-associated herpesvirus K-Rta exhibits SUMO-targeting ubiquitin

Izumiya Y, Kobayashi K, Kim KY, Pochampalli M, Izumiya C, Shevchenko B, Wang DH, Huerta SB, Martinez A, Campbell M, Kung HJ. PLoS Pathog. 2013;9(8):e1003506.

AXIN is an essential co-activator for the promyelocytic leukemia protein in p53 - AXIN is an essential co-activator for the promyelocytic leukemia protein in p53

Li Q, He Y, Wei L, Wu X, Wu D, Lin S, Wang Z, Ye Z, Lin SC. Oncogene. 2011 Mar 10;30(10):1194-204.