Anti-PPP1CA + 1CB antibody [EP1511Y]

• Supplier: Abcam
• Catalog: ab52619
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1511Y
• Applications: WB IP ICC/IF IHC-P FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues on human PP1 alpha
• Description: Rabbit Monoclonal
• Gene: PPP1CA
• Conjugate: Unconjugated

Product images

ab52619 (purified) at 1/30 immunoprecipitating PPP1CA + 1CB in Jurkat cell lysate. For western blotting, a HRP-conjugated Goat anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1000).Blocking buffer and concentration: 5% NFDM/TBST.Diluting buffer and concentration: 5% NFDM /TBST.

Overlay histogram showing HeLa cells stained with ab52619 (red line) at 1/150 dilution. The cells were fixed with 80% methanol. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)

ab52619 staining PPP1CA + 1CB in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

ab52619 staining PP1CA + 1CB in Human cerebrum cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/50). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

All lanes : Anti-PPP1CA + 1CB antibody [EP1511Y] (ab52619) at 1/100000 dilutionLane 1 : Mouse Brain lysateLane 2 : Rat Brain lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

All lanes : Anti-PPP1CA + 1CB antibody [EP1511Y] (ab52619) at 1/100000 dilutionLane 1 : T47-D cell LysateLane 2 : HeLa cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

All lanes : Anti-PPP1CA + 1CB antibody [EP1511Y] (ab52619) at 1/20000 dilutionLane 1 : SKBR-3 cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Anti-PPP1CA + 1CB antibody [EP1511Y] (ab52619) at 1/200000 dilution (unpurified) + HeLa cell lysate at 10 µgSecondaryGoat anti-rabbit HRP antibody at 1/2000 dilution

ab52619, unpurified, at 1/100 dilution staining Human uterus carcinoma tissue; paraffin embedded.Ab52619 at 1/100 dilution staining human uterus carcinoma tissue; paraffin embedded.

Overlay histogram showing HeLa cells stained with ab52619, unpurified (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52619, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

ICC/IF image of ab52619, unpurified, stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab52619 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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