Anti-Ret (phospho Y1062) antibody
| Name | Anti-Ret (phospho Y1062) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab51103 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | A synthetic phospho-peptide derived from human Ret around the phosphorylation site of tyrosine 1062 (K-L-YP-G-M) |
| Description | Rabbit Polyclonal |
| Gene | RET |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Ret (phospho Y1062) antibody (ab51103) at 1/500 dilutionLane 1 : K562 cell lysate.Lane 2 : K562 cell Lysate. For this panel, ab51103 was pre-incubated with immunizing peptide.
Ab51103 staining human normal testis tissue. Staining is localised to cell membranes.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.