Anti-Ret antibody [EPR2871]

• Supplier: Abcam
• Catalog: ab134100
• Prices: $391.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR2871
• Applications: ELISA WB IP IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide corresponding to residues on the C terminus of Human Ret (UniProt P07949)
• Description: Rabbit Monoclonal
• Gene: RET
• Conjugate: Unconjugated

Product images

All lanes : Anti-Ret antibody [EPR2871] (ab134100) at 1/10000 dilutionLane 1 : Neuro-2a cell lysateLane 2 : TT cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

All lanes : Anti-Ret antibody [EPR2871] (ab134100) at 1/10000 dilutionLane 1 : Mouse brainLane 2 : Rat brainLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labelling Ret with ab134100 at 1/250 dilution.

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Ret with ab134100 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, 1/500). Counter stained with hematoxylin.

Overlay histogram showing Caco 2 cells stained with ab134100 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134100, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Product References

TFAP2C governs the luminal epithelial phenotype in mammary development and - TFAP2C governs the luminal epithelial phenotype in mammary development and

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Kinase fusions are frequent in Spitz tumours and spitzoid melanomas. - Kinase fusions are frequent in Spitz tumours and spitzoid melanomas.

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Effects of siRNA on RET/PTC3 junction oncogene in papillary thyroid carcinoma: - Effects of siRNA on RET/PTC3 junction oncogene in papillary thyroid carcinoma:

Ali HM, Urbinati G, Chapuis H, Desmaele D, Bertrand JR, Couvreur P, Massaad-Massade L. PLoS One. 2014 Apr 23;9(4):e95964.

Comprehensive analysis of RET and ROS1 rearrangement in lung adenocarcinoma. - Comprehensive analysis of RET and ROS1 rearrangement in lung adenocarcinoma.

Lee SE, Lee B, Hong M, Song JY, Jung K, Lira ME, Mao M, Han J, Kim J, Choi YL. Mod Pathol. 2015 Apr;28(4):468-79.

FRAX597, a small molecule inhibitor of the p21-activated kinases, inhibits - FRAX597, a small molecule inhibitor of the p21-activated kinases, inhibits

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c-Jun in Schwann cells promotes axonal regeneration and motoneuron survival via - c-Jun in Schwann cells promotes axonal regeneration and motoneuron survival via

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