Anti-Ret antibody [RET01]

• Supplier: Abcam
• Catalog: ab1840
• Prices: $378.00
• Sizes: 500 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: RET01
• Applications: IHC-P IHC-F ICC/IF ICC/IF IHC-P
• Species Reactivities: Human
• Antigen: BALB/C mice were injected with recombinant protein encoding the extreme C-terminal cytoplasmic region of the human ret oncoprotein
• Description: Mouse Monoclonal
• Gene: RET
• Conjugate: Unconjugated

Product images

ab1840 - immunohistochemistry

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human intestine tissue, staining Ret with ab1840. Staining was detected using DAB.

ICC/IF image of ab1840 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1840, used neat (Undiluted)) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

Characterization of neuronal populations in the human trigeminal ganglion and - Characterization of neuronal populations in the human trigeminal ganglion and

Flowerdew SE, Wick D, Himmelein S, Horn AK, Sinicina I, Strupp M, Brandt T, Theil D, Hufner K. PLoS One. 2013 Dec 19;8(12):e83603.

Ret, GFRalpha-1, GFRalpha-2 and GFRalpha-3 receptors in the human hippocampus and - Ret, GFRalpha-1, GFRalpha-2 and GFRalpha-3 receptors in the human hippocampus and

Serra MP, Quartu M, Mascia F, Manca A, Boi M, Pisu MG, Lai ML, Del Fiacco M. Int J Dev Neurosci. 2005 Aug;23(5):425-38.