Anti-Ribophorin I antibody
| Name | Anti-Ribophorin I antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab86267 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Cat, Dog, Pig, Zebrafish |
| Antigen | Synthetic peptide within residues: AAEARMKVAC ITEQVLTLVN KRIGLYRHFD ETVNRYKQSR DISTLNSGKK , corresponding to internal sequence amino acids 467-516 of Human Ribophorin I (NP_002941) |
| Description | Rabbit Polyclonal |
| Gene | RPN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Ribophorin I antibody (ab86267) at 1 µg/ml + Transfected 293T cell lysate at 10 µgSecondaryanti-Rabbit IgG HRP at 1/50000 dilution
ICC/IF image of ab86267 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86267, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Ribophorin I antibody (ab86267) at 1 µg/mlLane 1 : HeLa cellsLane 2 : CHO-K1 celsLane 3 : Rough canine microsomes