RPN1

Gene Symbol RPN1
Entrez Gene 6184
Alt Symbol OST1, RBPH1
Species Human
Gene Type protein-coding
Description ribophorin I
Other Description RPN-I|dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit|dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1|dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit|oligosaccharyltransferase 1 homolog|oligosaccharyltransferase complex subunit (non-catalytic)|ribophorin-1
Swissprots P04843 Q68DT1 B2R5Z0 D3DNB6
Accessions CAH05344 CAH05363 EAW79306 EAW79307 EAW79308 EAW79309 P04843 AK223595 BAD97315 AK296905 BAG59461 AK297513 BAH12600 AK297941 BAG60257 AK312369 BAG35287 BC007995 AAH07995 BC010839 AAH10839 BF930946 BG720812 CR456742 CAG33023 CR749284 CAH18139 JF432331 ADZ15548 Y00281 CAA68392 NM_002950 NP_002941
Function Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Subcellular Location Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Specificity Expressed in all tissues tested. {ECO:0000269|PubMed:12887896}.
Top Pathways N-Glycan biosynthesis, Protein processing in endoplasmic reticulum

Ribophorin I (C-15) - sc-12164 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ribophorin I (C-15): sc-12164. Western blot analysis of Ribophorin I expression in non-transfected 293T: sc-117752 (A), human Ribophorin I transfected 293T: sc-113553 (B) and K-562 (C) whole cell lysates.

Ribophorin I (E-16) - sc-12163 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ribophorin I (E-16): sc-12163. Western blot analysis of Ribophorin I expression in non-transfected: sc-117752 (A) and human Ribophorin I transfected: sc-113553 (B) 293T whole cell lysates.

Ribophorin I (H-50) - sc-25559 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Ribophorin I (H-50): sc-25559. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining in exocrine pancreas at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Ribophorin I (E-7) - sc-48367 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ribophorin I (E-7): sc-48367. Western blot analysis of Ribophorin I expression in K-562 whole cell lysate.

Anti-Ribophorin I antibody - ab197819 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ribophorin I antibody (ab197819) at 1/350 dilutionLane 1 : HeLa cell lysateLane 2 : A549 cell lysateLane 3 : Human liver cancer tissue lysateLysates/proteins at 40 µg per lane.

Anti-Ribophorin I antibody - ab86267 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-Ribophorin I antibody (ab86267) at 1 µg/ml + Transfected 293T cell lysate at 10 µgSecondaryanti-Rabbit IgG HRP at 1/50000 dilution

Anti-Ribophorin I antibody - ab74725 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-Ribophorin I antibody (ab74725) at 1 µg/ml + MCF7 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Ribophorin I antibody - ab38451 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ribophorin I antibody (ab38451) at 1/100 dilutionLane 1 : HeLa cell lysateLane 2 : Mouse liver tissue lysate

Anti-Ribophorin I antibody - ab58336 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab58336 Ribophorin I antibody at 1ug/ml.

Anti-Ribophorin I antibody [3E1] - ab123904 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3E1
All lanes : Anti-Ribophorin I antibody [3E1] (ab123904) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Ribophorin I cDNALysates/proteins at 5 µg per lane.

Anti-Ribophorin I antibody [EPR17044(B)] - ab197888 from Abcam

Prices $347.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR17044(B)
All lanes : Anti-Ribophorin I antibody [EPR17044(B)] (ab197888) at 1/20000 dilutionLane 1 : BxPC-3 (human Pancreas adenocarcinoma ) whole cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-Ribophorin I antibody - C-terminal - ab137668 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Ribophorin I antibody - C-terminal (ab137668) at 1/1500 dilutionLane 1 : H1299 whole cell lysate Lane 2 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-RPN1 / Ribophorin I Antibody (clone 5B1) - LS-C173362 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5B1
IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RPN1 mouse monoclonal antibody.

Anti-RPN1 / Ribophorin I Antibody (clone 6F2) - LS-C173354 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6F2
Anti-RPN1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPN1.

Anti-RPN1 / Ribophorin I Antibody (clone 2C10-2F6) - LS-C198117 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2C10-2F6
Detection limit for recombinant GST tagged RPN1 is approximately 1 ng/ml as a capture antibody.

Anti-RPN1 / Ribophorin I Antibody (aa559-587, APC) - LS-C268512 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPN1 / Ribophorin I Antibody (aa559-587, AP) - LS-C268513 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPN1 / Ribophorin I Antibody (clone 2G9) - LS-C173360 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2G9
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPN1.

Anti-RPN1 / Ribophorin I Antibody (aa559-587, Biotin) - LS-C268514 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPN1 / Ribophorin I Antibody (aa559-587, FITC) - LS-C268515 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPN1 / Ribophorin I Antibody (aa559-587, Azide-free, HRP) - LS-C268516 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPN1 / Ribophorin I Antibody (aa559-587, PE) - LS-C268518 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPN1 / Ribophorin I Antibody (C-Terminus, APC) - LS-C282219 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPN1 / Ribophorin I Antibody (C-Terminus, Biotin) - LS-C282220 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RPN1 / Ribophorin I Antibody (C-Terminus, FITC) - LS-C282221 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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