Anti-Secretory phospholipase A2 Type V antibody
| Name | Anti-Secretory phospholipase A2 Type V antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab23709 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: CAIRTQSYDY RYTNGL conjugated to KLH, corresponding to amino acids 79-94 of Mouse Secretory phospholipase A2 Type V |
| Description | Rabbit Polyclonal |
| Gene | PLA2G5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC image of Secretory phospholipase A2 Type V staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23709, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Secretory phospholipase A2 Type V antibody (ab23709) at 1/500 dilutionLane 1 : Recombinant human sPLA2 (type V) (100 ng)Lane 2 : Recombinant human sPLA2 (type V) (50 ng)Lane 3 : Mouse heart homogenate (50ug)