Anti-Sonic Hedgehog antibody
| Name | Anti-Sonic Hedgehog antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab73958 |
| Prices | $382.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | A synthetic peptide corresponding to N-terminus of human Sonic Hedgehog |
| Description | Rabbit Polyclonal |
| Gene | SHH |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Human lung tissue stained with ab73958 at 1/100 dilution for 30 mins at RT. Tissue sections boiled in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
ICC/IF image of ab73958 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73958, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Sonic hedgehog improves redifferentiation of dedifferentiated chondrocytes for - Sonic hedgehog improves redifferentiation of dedifferentiated chondrocytes for
Lin L, Shen Q, Xue T, Duan X, Fu X, Yu C. PLoS One. 2014 Feb 12;9(2):e88550.
Heparan sulfate proteoglycans containing a glypican 5 core and 2-O-sulfo-iduronic - Heparan sulfate proteoglycans containing a glypican 5 core and 2-O-sulfo-iduronic
Witt RM, Hecht ML, Pazyra-Murphy MF, Cohen SM, Noti C, van Kuppevelt TH, Fuller M, Chan JA, Hopwood JJ, Seeberger PH, Segal RA. J Biol Chem. 2013 Sep 6;288(36):26275-88.
Role of Sonic Hedgehog in idiopathic pulmonary fibrosis. - Role of Sonic Hedgehog in idiopathic pulmonary fibrosis.
Bolanos AL, Milla CM, Lira JC, Ramirez R, Checa M, Barrera L, Garcia-Alvarez J, Carbajal V, Becerril C, Gaxiola M, Pardo A, Selman M. Am J Physiol Lung Cell Mol Physiol. 2012 Dec 1;303(11):L978-90. doi: