Western blot analysis of extracts from various cell lines using CREB (D76D11) Rabbit mAb.
Confocal immunofluorescent analysis of mouse brain using CREB (D76D11) Rabbit mAb (green) and β3-Tubulin (TU-20) Mouse mAb #4466 (red).
Confocal immunofluorescent analysis of SH-SY5Y cells using CREB (D76D11) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Flow cytometric analysis of SH-SY5Y cells, using CREB (D76D11) Rabbit mAb Antibody (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 293 cells treated with Forskolin #3828 (30 µM) and either 10 μl of CREB (D76D11) XP ® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ALS2 exon 1 primers, SimpleChIP ® Human Nr4A3 Promoter Primers #4829, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.