Anti-VEGF Receptor 1 antibody [Y103]
| Name | Anti-VEGF Receptor 1 antibody [Y103] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32152 |
| Prices | $404.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | Y103 |
| Applications | WB IHC-P ICC/IF ICC/IF IP FC IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues in N terminus of human VEGF Receptor 1 |
| Blocking Peptide | VEGF Receptor 1 peptide |
| Description | Rabbit Monoclonal |
| Gene | FLT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing A431 cells stained with ab32152 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32152, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used a goat anti-rabbit DyLight® 488 goat (IgG, H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/10000 dilution + mouse brain tissue](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19487_Wb%2520VEGFR1.jpg)
Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/10000 dilution + mouse brain tissue
Immunofluorescent analysis of VEGF receptor 1 expression in A431 cells using 1/250 ab32152.
Immunohistochemical analysis of VEGF receptor 1 expression in paraffin-embedded skin cancer, using 1/250 ab32152.Immunohistochemical analysis of VEGF receptor 1 expression in paraffin-embedded skin cancer, using 1/250 ab32152.
ab32152 at 1/200 dilution staining VEGF Receptor 1 in human 293FT cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and blocked in 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/200 dilution in PBS and incubated with sample at 4°C for 12 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG was used as secondary at 1/500 dilution.See Abreview
Product References
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