
Western Blotting Analysis:
Representative lot data.
Proteins in mouse retina post-nuclear lysate were seperated by electrophoresis (~25 µg or ~0.3 retina equivalent per lane) without prior heating, transferred to nitrocellulose membrane, blocked with 5% milk in TBST, and probed with Cat. No. MABN1841, Anti-TRPM1, clone 545H5 (1 µg/mL) overnight at 4°C. Proteins were visualized using an anti-mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates TRPM1 (~180 kDa).
(Image courtesy of Dr. Melina A. Agosto, Baylor College of Medicine, Houston, TX)