GLI-2 in Mouse Embryo. GLI-2 was detected in immersion fixed frozen sections of mouse embryo (15 d. p. c.) using Goat Anti-Mouse GLI-2 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing muscle.