GLI2

Gene Symbol GLI2
Entrez Gene 2736
Alt Symbol CJS, HPE9, PHS2, THP1, THP2
Species Human
Gene Type protein-coding
Description GLI family zinc finger 2
Other Description GLI family zinc finger protein 2|GLI-Kruppel family member GLI2|glioma-associated oncogene family zinc finger 2|oncogene GLI2|tax helper protein 1|tax helper protein 2|tax-responsive element-2 holding protein|tax-responsive element-25-bp sequence binding protein|zinc finger protein GLI2
Swissprots O60255 O60252 Q4JHT4 O60254 Q15591 O60253 P10070 Q15590
Accessions AAA35898 P10070 AB007295 BAA25665 AB007296 BAA25666 AB007297 BAA25667 AB007298 BAA25668 AB209354 BAD92591 AJ707583 AK300071 BAG61875 AY493737 AAS72889 AY493738 AAS72890 AY493739 AAS72891 BC111410 AAI11411 BC172434 AAI72434 D14827 BAA03568 D14828 BAA03569 DQ004396 AAY58315 DQ004397 AAY58316 DQ004398 AAY58317 DQ086814 AAY87165 EF547128 XM_006712422 XP_006712485 XM_011510969 XP_011509271 XM_011510970 XP_011509272 XM_011510971 XP_011509273 XM_011510972 XP_011509274 XM_011510973 XP_011509275 XM_011510974 XP_011509276 NM_005270 NP_005261 NM_030379 NM_030380 NM_030381
Function Isoform 5: Acts as a transcriptional repressor. {ECO:0000269|PubMed:15994174}.
Subcellular Location Isoform 2: Nucleus {ECO:0000269|PubMed:9557682}.
Tissue Specificity Isoform 1 and isoform 4 are expressed in HTLV- 1-infected T-cell lines (at protein level). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines. {ECO:0000269|PubMed:9557682}.
Top Pathways Hedgehog signaling pathway, Basal cell carcinoma, Hippo signaling pathway

GLI2 (R770) Antibody - 2585 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of COS cell extracts, untransfected or transiently transfected with a construct expressing GLI2, using GLI2 (R770) Antibody.

GLI-2 (C-10) - sc-271786 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GLI-2 (C-10): sc-271786. Western blot analysis of GLI-2 expression in F9 (A) and K-562 (B) whole cell lysates.

GLI-2 (G-20) - sc-20291 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GLI-2 (G-20): sc-20291. Western blot analysis of GLI-2 expression in HOS (A), K-562 (B) and TF-1 (C) whole cell lysates.

GLI-2 (H-300) - sc-28674 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GLI-2 (H-300): sc-28674. Western blot analysis of GLI-2 expression in K-562 (A), TF-1 (B), PC-3 (C), Jurkat (E) and MDA-MB-231 (F) nuclear extracts and F9 whole cell lysate (D).

GLI-2 (N-20) - sc-20290 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GLI-2 (N-20): sc-20290. Western blot analysis of GLI-2 expression in non-transfected: sc-117752 (A) and human GLI-2 transfected: sc-372196 (B) 293T whole cell lysates.

Anti-Gli2 antibody - ab7181 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-Gli2 antibody - C-terminal - ab199933 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Gli2 antibody - C-terminal (ab199933) at 1/500 dilution + Jurkat total cell extract

Anti-Gli2 antibody - ChIP Grade - ab26056 from Abcam

Prices $400.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Gli2 antibody - ChIP Grade (ab26056) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate 10ug.SecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-GLI2 Antibody (aa729-1123, clone 1G1) - LS-C339242 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1G1
IHC of paraffin-embedded Human testicular cancer tissue using anti-GLI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-GLI2 Antibody (aa1444-1463) - LS-C313075 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
WB of GLI2 antibody. Lane 1: MCF-7 Cell Lysate. Lane 2: HELA Cell Lysate. Lane 3: SKOV Cell Lysate. Lane 4: HT1080 Cell Lysate. Lane 5: A549 Cell Lysate..

Anti-GLI2 Antibody (aa46-60) IHC-plus™ - LS-B2808 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GLI2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2808 concentration 5 ug/ml.

Anti-GLI2 Antibody (aa1287-1321) - LS-C344252 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Fluorescent image of HeLa cells stained with GLI2 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Anti-GLI2 Antibody (Internal) IHC-plus™ - LS-B75 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-GLI2 antibody IHC of mouse, brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B75 concentration 5 ug/ml.

Anti-GLI2 Antibody (aa416-429) - LS-C54915 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-GLI2 Antibody (Internal) - LS-C121874 from LifeSpan Bioscience

Prices $695.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-GLI2 Antibody (aa729-1123, clone 1B2) - LS-C339225 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2.

Anti-GLI2 Antibody (aa729-1123, clone 1G2) - LS-C339241 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1G2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GLI2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GLI2.

Anti-GLI2 Antibody (aa729-1123, clone 1F9) - LS-C339221 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1F9
IHC of paraffin-embedded Human skin tissue using anti-GLI2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-GLI2 Antibody - LS-C149960 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal
Detection of GLI-2-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/ml PMA and 200 ng/ml calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-2/DNA complexes were immunoprecipitated using 5 ug Sheep Anti-Human GLI-2 Antigen Affinity-purified Polyclonal Antibody or control antibody for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Sheep IgG Secondary Antibody. immune complexes were captured using 50 ul of MagCellect Streptavidin Ferrofluid and DNA was purified using chelating resin solution. The bcl-2 promoter was detected by standard PCR.

Anti-GLI2 Antibody (aa1-416) - LS-C150201 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
GLI-2 in Mouse Embryo. GLI-2 was detected in immersion fixed frozen sections of mouse embryo (15 d. p. c.) using Goat Anti-Mouse GLI-2 Antigen Affinity-purified Polyclonal Antibody at 15 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing muscle.

Anti-GLI2 Antibody (aa600-649) - LS-C31484 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
GLI2 antibody LS-C31484 was used in IHC to stain formalin-fixed, paraffin-embedded human intestine.

GLI-2 Antibody - NBP2-23602 from Novus Biologicals

Prices $99.00, $299.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: GLI-2 Antibody [NBP2-23602] - Gli2 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilution of 1:50 was used. Image objective 40x.

GLI-2 Antibody - NB600-874 from Novus Biologicals

Prices $129.00, $399.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: GLI-2 Antibody [NB600-874] - Analysis of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). See Ruppert et al for testing conditions. Each lane contains approximately 35 ug of lysate. Primary antibody was used at a 1:400 dilution in 5% BLOTTO in PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at room temperature (800 nm channel, green).

GLI-2 Antibody - AF3526 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal

GLI-2 Antibody - NB100-2815 from Novus Biologicals

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Immunohistochemistry-Paraffin: GLI-2 Antibody [NB100-2815] - Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.
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