![Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TPA and UV-treated, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb (upper) or MEK1/2 Antibody #9122 (lower).](http://www.bioprodhub.com/system/product_images/ab_products/1/sub_1/25313_2338_wb_ka_050705.jpg)
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TPA and UV-treated, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb (upper) or MEK1/2 Antibody #9122 (lower).
![Immunohistochemical analysis of formalin fixed, paraffin-embedded A375 cells, untreated (left) or treated with Raf1 Kinase Inhibitor 1 (right), using Phospho-MEK1/2 (Ser 221) (166F8) Rabbit mAb.](http://www.bioprodhub.com/system/product_images/ab_products/1/sub_1/25314_2338_ihc_ka.jpg)
Immunohistochemical analysis of formalin fixed, paraffin-embedded A375 cells, untreated (left) or treated with Raf1 Kinase Inhibitor 1 (right), using Phospho-MEK1/2 (Ser 221) (166F8) Rabbit mAb.
![Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-MEK1/2 (Ser221)(166F8) Rabbit mAb.](http://www.bioprodhub.com/system/product_images/ab_products/1/sub_1/25315_2354_ihc_bt_ka_050107.jpg)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-MEK1/2 (Ser221)(166F8) Rabbit mAb.
![Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb.](http://www.bioprodhub.com/system/product_images/ab_products/1/sub_1/25316_2354_ihc_lung_ka_050107.jpg)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb.
![Immunohistochemical analysis of paraffin-embedded prostate carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb.](http://www.bioprodhub.com/system/product_images/ab_products/1/sub_1/25317_2354_ihc_pros_ka_050107.jpg)
Immunohistochemical analysis of paraffin-embedded prostate carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb.
![Flow cytometric analysis of Jurkat cells, untreated (blue) or PMA-treated (green), using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb compared to a nonspecific negative control antibody (red).](http://www.bioprodhub.com/system/product_images/ab_products/1/sub_1/25318_2338_fc_ka_050705.jpg)
Flow cytometric analysis of Jurkat cells, untreated (blue) or PMA-treated (green), using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb compared to a nonspecific negative control antibody (red).