MAP2K1

Gene Symbol MAP2K1
Entrez Gene 5604
Alt Symbol CFC3, MAPKK1, MEK1, MKK1, PRKMK1
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase kinase 1
Other Description ERK activator kinase 1|MAPK/ERK kinase 1|MAPKK 1|MEK 1|dual specificity mitogen-activated protein kinase kinase 1|protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Swissprots Q02750
Accessions EAW77765 EAW77766 EAW77767 EAW77768 EAW77769 Q02750 AK291500 BAF84189 AK294322 BAG57596 AK312356 BC137459 AAI37460 BC139729 AAI39730 BE856741 BG709050 CA419665 DA653983 L05624 AAA36318 L11284 XM_011521783 XP_011520085 NM_002755 NP_002746
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of th
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
Tissue Specificity Widely expressed, with extremely low levels in brain. {ECO:0000269|PubMed:1281467}.
Top Pathways VEGF signaling pathway, Focal adhesion, Gap junction, Toll-like receptor signaling pathway, Natural killer cell mediated cytotoxicity

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) - 3958 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 41G9
Western blot analysis of extracts of HeLa cells, untreated or TPA-treated, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Biotinylated) and Streptavidin-HRP (#3999).

MEK1/2 (D1A5) Rabbit mAb (HRP Conjugate) - 13033 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1A5
Western blot analysis of extracts from NIH/3T3 and HeLa cells using MEK1/2 (D1A5) Rabbit mAb (HRP Conjugate).

MEK1 (61B12) Mouse mAb (Biotinylated) - 5744 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 61B12
Western blot analysis of extracts from various cell lines using MEK1 (61B12) Mouse mAb (Biotinylated).

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 5068 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 41G9
Flow cytometric analysis of Jurkat cells, treated with U0126 (MEK1/2 Inhibitor) #9903 (blue) or TPA (Phorbol-12-Myristate-13-Acetate) #9905 (green), using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Alexa Fluor ® 647 Conjugate).

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 4660 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 41G9
Flow cytometric analysis of Jurkat cells, treated with U0126 (MEK1/2 Inhibitor) #9903 (blue) or TPA (Phorbol-12-Myristate-13-Acetate) #9905 (green), using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (Alexa Fluor ® 488 Conjugate).

PhosphoPlus® MEK1/2 (Ser217/221) Antibody Kit - 9120 from Cell Signaling Technology

Prices $489.00
Sizes 1 kit (10 western blots)
Clonality Polyclonal

Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb - 2338 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 166F8
Western blot analysis of extracts from HeLa and NIH/3T3 cells, untreated or TPA and UV-treated, using Phospho-MEK1/2 (Ser221) (166F8) Rabbit mAb (upper) or MEK1/2 Antibody #9122 (lower).

Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb - 9154 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 41G9
Western blot analysis of extracts from untreated or TPA-treated HeLa and NIH/3T3 cells, using Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb (upper), or MEK1/2 Antibody #9122 (lower).

Phospho-MEK1/2 (Ser217/221) Antibody - 9121 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from untreated, TPA-treated or lambda phosphatase-treated HeLa cells, using Phospho-MEK 1/2 (Ser217/221) Antibody #9121 (upper) or MEK 1/2 Antibody #9122 (lower).

Phospho-MEK1 (Ser298) Antibody - 9128 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from C2C12, C6 and COS cells, untreated or PMA-treated, using Phospho-MEK1 (Ser298) Antibody (upper) or MEK1 Antibody #9124 (lower). In lane 3, the C2C12 lysate was treated with alkaline phosphatase (CIP) to show the phospho-specificity of the antibody.

Phospho-MEK1 (Thr286) Antibody - 9127 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A431, COS and PC12 cells, untreated or nocodazole-treated, using Phospho-MEK1 (Thr286) Antibody (upper) or MEK1 Antibody #9124 (lower).

MEK1/2 (D1A5) Rabbit mAb - 8727 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1A5
Western blot analysis of extracts from various cell lines using MEK1/2 (D1A5) Rabbit mAb.

MEK1/2 Antibody - 9122 from Cell Signaling Technology

Prices $246.00, $596.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from untreated, TPA-treated or lambda phosphatase-treated HeLa cells, using Phospho-MEK 1/2 (Ser217/221) Antibody #9121 (upper) or MEK 1/2 Antibody #9122 (lower).

MEK1/2 (L38C12) Mouse mAb - 4694 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone L38C12
Western blot analysis of extracts from NIH/3T3, PC12 and COS cells, using MEK1/2 (L38C12) Mouse mAb.

MEK1 Antibody - 9124 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using MEK1 Antibody.

MEK1 (D2R1O) Rabbit mAb - 12671 from Cell Signaling Technology

Prices $250.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2R1O
Western blot analysis of extracts from various cell lines using MEK1 (D2R1O) Rabbit mAb (upper) or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower). MEK1/2 WT (MEK-WT), MEK1 knockout (MEK1-/-) and MEK2 knockout (MEK2-/-) MEFs were generously provided by Dr. Jean Charron, Centre de recherche du Centre hospitalier de l'Université Laval, Quebec, Canada.

MEK1/2 (47E6) Rabbit mAb - 9126 from Cell Signaling Technology

Prices $256.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 47E6
Western blot analysis of extracts from HeLa, NIH/3T3, COS and PC12 cells, using MEK1/2 (47E6) Rabbit mAb.

MEK1 (30C8) Rabbit mAb - 9146 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 30C8
Western blot analysis of extracts from HeLa, NIH/3T3 and COS cells, using MEK1 (30C8) Rabbit mAb.

MEK1 (61B12) Mouse mAb - 2352 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 61B12
Western blot analysis of extracts from HeLa, NIH/3T3 and A431 cells, using MEK1 (61B2) Mouse mAb.

Phospho-MEK1/2(Ser217/221) Antibody - 5130 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-MEK1/2 (Ser217/221) (166F8) Rabbit mAb - 2354 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 166F8

MEK-1 (A-8) - sc-365800 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MEK-1 (A-8): sc-365800. Western blot analysis of MEK-1 expression in KNRK whole cell lysate.

MEK-1 (C-18) - sc-219 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MEK-1 (C-18): sc-219. Western blot analysis of MEK-1 expression in KNRK (A) and CTLL-2 (B) whole cell lysates.

MEK-1 (H-8) - sc-6250 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MEK-1 (H-8): sc-6250. Western blot analysis of MEK-1 expression in AT-3 (A), KNRK (B) and HeLa (C) whole cell lysates.

MEK-1/2 (12-B) - sc-436 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MEK-1/2 (12-B): sc-436. Western blot analysis of MEK-1 and MEK-2 expression in KNRK whole cell lysate.
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