MAP2K1

Gene Symbol MAP2K1
Entrez Gene 5604
Alt Symbol CFC3, MAPKK1, MEK1, MKK1, PRKMK1
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase kinase 1
Other Description ERK activator kinase 1|MAPK/ERK kinase 1|MAPKK 1|MEK 1|dual specificity mitogen-activated protein kinase kinase 1|protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
Swissprots Q02750
Accessions EAW77765 EAW77766 EAW77767 EAW77768 EAW77769 Q02750 AK291500 BAF84189 AK294322 BAG57596 AK312356 BC137459 AAI37460 BC139729 AAI39730 BE856741 BG709050 CA419665 DA653983 L05624 AAA36318 L11284 XM_011521783 XP_011520085 NM_002755 NP_002746
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of th
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body. Cytoplasm. Nucleus. Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis.
Tissue Specificity Widely expressed, with extremely low levels in brain. {ECO:0000269|PubMed:1281467}.
Top Pathways VEGF signaling pathway, Focal adhesion, Gap junction, Toll-like receptor signaling pathway, Natural killer cell mediated cytotoxicity

PathScan ® Phospho-MEK1 (Ser217/221) Sandwich ELISA Antibody Pair - 7211 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between lysate protein concentration from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Antibody Pair #7211 is shown. NIH/3T3 cells were treated with PDGF for 20 minutes at 37ºC and then lysed.

PathScan ® Phospho-MEK1 (Ser217/221) Chemiluminescent Sandwich ELISA Kit - 7029 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Relationship between protein concentration of lysates from untreated and PDGF-treated NIH/3T3 cells and immediate light generation with chemiluminescent substrate is shown. Cells (80% confluence) were treated with PDGF (50 ng/ml) and lysed after incubation at 37ºC for 5 minutes. Graph inset corresponding to the shaded area shows high sensitivity and a linear response at the low protein concentration range.

PathScan® Phospho-MEK1 (Ser217/221) Sandwich ELISA Kit - 7175 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)

PathScan ® Total MEK1 Sandwich ELISA Antibody Pair - 7215 from Cell Signaling Technology

Category Antibody Pair
Prices $459.00
Sizes 1 kit (Reagents for 4 x 96 well plates)
The relationship between lysate protein concentration from untreated and PDGF-treated NIH/3T3 cells and the absorbance at 450 nm using PathScan® Total MEK1 Sandwich ELISA Antibody Pair #7215 is shown. NIH/3T3 cells were treated with PDGF for 20 minutes at 37ºC and then lysed.

PathScan® Total MEK1 Sandwich ELISA Kit - 7165 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)

PathScan ® Total MEK1 Chemiluminescent Sandwich ELISA Kit - 7030 from Cell Signaling Technology

Category ELISA Kit

Mek1 (pS217/221) + total Mek1 ELISA Kit - ab126448 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
HeLa cells were treated with TPA at 37°C for 15 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of cell lysates were analyzed using ab126448.

Mek1 (pS217/221) ELISA Kit - ab126446 from Abcam

Category ELISA Kit
Prices $489.00
Sizes 1 x 96 tests

Mouse/Human/Rat Phospho-MAP2K1 / MKK1 / MEK1 (Thr286) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1260 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate
Range 0.156-10 ng/ml

Mouse/Human/Rat Phospho-MAP2K1 / MKK1 / MEK1 (Ser298) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1259 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate
Range 0.156-10 ng/ml

Mouse/Human/Rat MAP2K1 / MKK1 / MEK1 ELISA Kit (Cell-Based ELISA) - LS-F2139 from LifeSpan Bioscience

Category ELISA Kit
Prices $450.00
Sizes 1 plate
Range 0.156-10 ng/ml

Mouse/Human/Rat Phospho-MAP2K1 / MKK1 / MEK1 (Thr291) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1561 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate
Range 0.156-10 ng/ml

Human MAP2K1 / MKK1 / MEK1 ELISA Kit (Sandwich ELISA) - LS-F7448 from LifeSpan Bioscience

Category ELISA Kit
Prices $720.00
Sizes 1 plate
Sensitivity 0.056 ng/ml
Range 0.156-10 ng/ml

Human MAP2K1 / MKK1 / MEK1 ELISA Kit (Sandwich ELISA) - LS-F12075 from LifeSpan Bioscience

Category ELISA Kit
Prices $530.00
Sizes 1 plate
Sensitivity 0.156
Range 0.156-10 ng/ml
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Rabbit anti-Phospho MEK1(T286) Antibody, Affinity Purified - PLA0285 from Sigma-Aldrich

Prices $345.00
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Phosphotyrosine MAP2K1 ELISA Kit - RAB0979 from Sigma-Aldrich

Category ELISA Kit
Prices $637.00
Sizes 1 kt

Phospho-Mek (pSer217 / 221) ELISA Kit - RAB0346 from Sigma-Aldrich

Category ELISA Kit
Prices $522.00
Sizes 1 kt

Phospho-Mek (pSer217 / 221) and pan-Mek ELISA Kit - RAB0347 from Sigma-Aldrich

Category ELISA Kit
Prices $570.00
Sizes 1 kt

Human MAP2K1 ELISA Kit - abx152266 from Abbexa

Category ELISA Kit
Sensitivity 0.056 ng/ml
Range 0.156 ng/ml - 10 ng/ml

Dual specificity mitogen-activated protein kinase kinase 1, MAP2K1, Human, ELISA Kit - E10995h from ARP American Research Products

Category ELISA Kit
Prices $476.00
Sizes 96 t
Range 0.156-10 ng/mL

Mitogen Activated Protein Kinase Kinase 1, MAP2K1, Human, ELISA Kit - E-EL-H0505 from ARP American Research Products

Category ELISA Kit
Prices $522.00
Sizes 96 t
Sensitivity 0.094
Range 0.156-10ng/mL

MEK1 (Phospho), Human, ELISA - IB09643 from ARP American Research Products

Category ELISA Kit
Prices $773.00
Sizes 96 t

MEK1 (Total), Human, ELISA - IB09644 from ARP American Research Products

Category ELISA Kit
Prices $625.00
Sizes 96 t

Mitogen Activated Protein Kinase 12 (MAPK12), Human, ELISA Kit - SED577Hu from ARP American Research Products

Category ELISA Kit
Prices $720.00
Sizes 96 t
Sensitivity 0.072ng/mL
Range 0.156-10ng/mL
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