Detection of Human Exportin-T by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Exportin-T antibody used for IP at 6 ug/mg lysate. Exportin-T was inefficiently immunoprecipitated by rabbit anti-Exportin-T antibody A303-972.A. For blotting immunoprecipitated Exportin-T, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human Exportin-T by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Exportin-T antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.