HIF-1 alpha Antibody

• Supplier: Novus Biologicals
• Catalog: NB100-479
• Prices: $149.00, $349.00
• Sizes: 25 µl, 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB ChIP FC ICC/IF IHC IHC-F IHC-P IP
• Species Reactivities: Human, Rat, Goat, Rabbit
• Antigen: A fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [UniProt# Q61221].
• Purity/Format: Immunogen affinity purified
• Description: Rabbit Polyclonal
• Gene: Hif1a
• Conjugate: Unconjugated

Product images

Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis of HIF-1 alpha in mouse hepatocytes (left lane: normoxic and right lane: hypoxic) using HIF-1 alpha antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Staining in normal mouse kidney showing (A) staining with antibody and (B) blocking peptide used at 10-fold excess concentration. Images provided by Dr Yves Heremans. Please see the product review of NBC1-18422 for additional details.

Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (50 ug, hypoxic). Lane 2: Untreated Cos-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure.

Immunohistochemistry: HIF-1 alpha Antibody [NB100-479] - Staining of placenta, villi.

Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [NB100-479] - Detection of HIF-1 Alpha (green) in RCC4 cells using NB100-479. Nuclei (Blue) were counterstained using Hoechst 33258.

Western Blot: HIF-1 alpha Antibody [NB100-479] - Analysis in A. PC12 (rat) cells, 20% oxygen and B. PC12 (rat) cells, 1% oxygen.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Analysis in paraffin section of mouse skin wound using NB100-479 at 1:6,000. Image courtesy of product review by Ian Darby of RMIT University.

Immunohistochemistry: HIF-1 alpha Antibody [NB100-479] - Analysis in normal mouse kidney. Image courtesy of product review by Yves Heremans.

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-479] - Analysis in human kidney tumor.