Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE‑2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ACE‑2 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE‑2 at approximately 141 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
ACE‑2 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.